Abstract
The brown planthopper (Nilaparvata lugens Stål, BPH) is the most destructive pest of rice (Oryza sativa L.). Utilizing resistant rice cultivars that harbor resistance gene/s is an effective strategy for integrated pest management. Due to the co-evolution of BPH and rice, a single resistance gene may fail because of changes in the virulent BPH population. Thus, it is urgent to explore and map novel BPH resistance genes in rice germplasm. Previously, an indica landrace from India, Paedai kalibungga (PK), demonstrated high resistance to BPH in both in Wuhan and Fuzhou, China. To map BPH resistance genes from PK, a BC1F2:3 population derived from crosses of PK and a susceptible parent, Zhenshan 97 (ZS97), was developed and evaluated for BPH resistance. A novel BPH resistance locus, BPH39, was mapped on the short arm of rice chromosome 6 using next-generation sequencing-based bulked segregant analysis (BSA-seq). BPH39 was validated using flanking markers within the locus. Furthermore, near-isogenic lines carrying BPH39 (NIL-BPH39) were developed in the ZS97 background. NIL-BPH39 exhibited the physiological mechanisms of antibiosis and preference toward BPH. BPH39 was finally delimited to an interval of 84 Kb ranging from 1.07 to 1.15 Mb. Six candidate genes were identified in this region. Two of them (LOC_Os06g02930 and LOC_Os06g03030) encode proteins with a similar short consensus repeat (SCR) domain, which displayed many variations leading to amino acid substitutions and showed higher expression levels in NIL-BPH39. Thus, these two genes are considered reliable candidate genes for BPH39. Additionally, transcriptome sequencing, DEGs analysis, and gene RT-qPCR verification preliminary revealed that BPH39 may be involved in the jasmonic acid (JA) signaling pathway, thus mediating the molecular mechanism of BPH resistance. This work will facilitate map-based cloning and marker-assisted selection for the locus in breeding programs targeting BPH resistance.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Funding
This work was supported in part by the National Natural Science Foundation of China (32025031, U1905203), Fujian Provincial Natural Science Foundation (2021J01131016), National Key Research and Development Program of China (2023YFF1000500), Fujian Agriculture and Forestry University School Innovation Fund (KFA20006A), and The Joint Open Competitive Project of the Yazhou Bay Seed Laboratory and China National Seed Company Limited (B23YQ1514, B23CQ15EP).
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Yangdong Ye and Yanan Wang performed experiments and analyzed the data, Ling Zou, Fangming Zhang, Shangye **ong, Baohui Liang, Cheng Chen and Zhihong Zhu collected plant materials and performed parts of experiments, Shuai Zhang, **aoqing Wu, and Weiren Wu participated in handling figures and tables, Jie Hu, Jianguo Wu and Shuai Zhang, the co-correspondence authors, designed this research and wrote the manuscript. All authors have approved the manuscript.
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Ye, Y., Wang, Y., Zou, L. et al. Identification and candidate analysis of a new brown planthopper resistance locus in an Indian landrace of rice, paedai kalibungga. Mol Breeding 44, 45 (2024). https://doi.org/10.1007/s11032-024-01485-6
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DOI: https://doi.org/10.1007/s11032-024-01485-6