Abstract
The gelatinization temperature of rice is an important factor in determining the eating and cooking quality, and it affects consumer preference. The alkali digestion value (ADV) is one of the main methods used to test the quality of rice and has a high correlation with the gelatinization temperature. For the development of high-quality rice, it is important to understand the genetic basis of palatability-related traits, and QTL analysis is a statistical method linking phenotypic data and genotype data and is an effective method to explain the genetic basis of variation in complex traits. QTL map** related to the ADV of brown and milled rice was performed using the 120 Cheongcheong/Nagdong double haploid (CNDH) line. As a result, 12 QTLs related to ADV were detected, and 20 candidate genes were selected from the RM588–RM1163 region of chromosome 6 through screening by gene function analysis. The comparison of the relative expression level of candidate genes showed that OsSS1q6 is highly expressed in CNDH lines with high ADV in both brown rice and milled rice. In addition, OsSS1q6 has high homology with the starch synthase 1 protein and interacts with various starch biosynthesis-related proteins, such as GBSSII, SBE, and APL. Therefore, we suggest that OsSS1q6 identified through QTL map** could be one of the various genes involved in the gelatinization temperature of rice by regulating starch biosynthesis. This study can be used as basic data for breeding high-quality rice and provides a new genetic resource that can increase the palatability of rice.
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This work was supported by a grant from the Low-Carbon Green Rice Production Technology Development Program (Project No. PJ01700602), Rural Development Administration, Republic of Korea.
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Y. -H. J., J. -R. P., and E. -G. K. conceptualized and wrote the manuscript. M. F. and D. D. Z. performed the experiments and collected the data. R. J., S. A., and K. -M. K revised the manuscript. K. -M. K. supervised this study. All authors read and approved the final manuscript.
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Jang, YH., Park, JR., Kim, EG. et al. Efficient identification of palatability-related genes using QTL map** in rice breeding. Mol Breeding 43, 39 (2023). https://doi.org/10.1007/s11032-023-01392-2
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DOI: https://doi.org/10.1007/s11032-023-01392-2