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Characterization of qPL5: a novel quantitative trait locus (QTL) that controls panicle length in rice (Oryza sativa L.)

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Abstract

Panicle length (PL) is an important trait that determines panicle architecture and strongly affects grain yield and quality in rice. However, this trait has not been well characterized genetically, and its contribution to yield improvement is not well understood. Characterization of novel genes related to PL is of great significance for breeding high-yielding rice varieties. In our previous research, we identified qPL5, a quantitative trait locus for PL. In this study, we aimed to determine the exact position of qPL5 in the rice genome and identify the candidate gene. Through substitution map**, we mapped qPL5 to a region of 21.86 kb flanked by the molecular marker loci STS5-99 and STS5-106 in which two candidate genes were predicted. By sequence analysis and relative expression analysis, LOC-Os05g41230, which putatively encodes a BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 precursor, was considered to be the most likely candidate gene for qPL5. In addition, we successfully developed a pair of near-isogenic lines (NILs) for qPL5 in different genetic backgrounds to evaluate the genetic effects of qPL5. Agronomic trait analysis of the NILs indicated that qPL5 positively contributes to plant height, grain number per panicle, panicle length, grain yield per plant, and flag leaf length, but it had no influence on heading date and grain-size-related traits. Therefore, qPL5 and the markers tightly linked to it should be available for molecular breeding of high-yielding varieties.

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Funding

This study was financially supported by the Jiangsu Province Government (Grant Nos. BK20200927, BE2022336, BE2021301-1, and PAPD program), and the National Natural Science Foundation (Grant Nos. 32001517 31771913 and 3207150123).

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Author notes

  1. Z. Xu and M. Li contributed equally to this paper.

Authors and Affiliations

  1. Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou, 225009, China

    Zuopeng Xu, Meng Li, Yuanyue Du, **xu Li, Ruixuan Wang, Zhiai Chen, Shuzhu Tang, Qiaoquan Liu & Honggen Zhang

  2. Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China

    Zuopeng Xu, Shuzhu Tang, Qiaoquan Liu & Honggen Zhang

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  1. Zuopeng Xu
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  2. Meng Li
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  4. **xu Li
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Contributions

ZX and HZ analyzed the data and drafted the manuscript. ZX performed the phenotypic evaluation and data analysis with assistance from ML, YD, XL, and RW. ZC and XZ participated in the construction of the SLs. ST participated in the design of the study. QL and HZ designed the study and revised the manuscript. All of the authors have read and approved the final version of the manuscript.

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Correspondence to Qiaoquan Liu or Honggen Zhang.

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Xu, Z., Li, M., Du, Y. et al. Characterization of qPL5: a novel quantitative trait locus (QTL) that controls panicle length in rice (Oryza sativa L.). Mol Breeding 42, 70 (2022). https://doi.org/10.1007/s11032-022-01339-z

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