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QTL map** for tuberous stem formation of kohlrabi (Brassica oleracea var. gongylodes L.)

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Abstract

The tuberous stem of kohlrabi is an important quantitative trait, which affects its yield and quality. Genetic control of this trait has not yet been unveiled. To identify the QTLs controlling stem swelling of kohlrabi, a BC1 population of 92 plants was developed from a cross of broccoli DH line GCP04 and kohlrabi var. Seine. A wide range of variation in tuberous stem diameter was observed among the map** populations. We constructed a genetic map of nine linkage groups (LGs) with different types of markers, spanning a total length of 913.5 cM with an average marker distance of 7.55 cM. Four significant QTLs for radial enlargement of kohlrabi stem, namely, REnBo1, REnBo2, REnBo3, and REnBo4 were detected on C02, C03, C05, and C09, respectively, and accounted for the phenotypic variation of 59% for the stem diameter and 55% for the qualitative grading of tuberous stem in classes. Then, we confirmed the stability of identified QTLs using BC1S1 populations derived from the BC1 plants having heterozygous alleles at the target QTL and homozygous kohlrabi alleles at the remaining QTLs. REnBo1and REnBo2 using 128 plants of BC168S1 and 94 plants of BC143S1, respectively, and REnBo3 and REnBo4 using 152 plants of BC157S1 were detected at the same positions as the respective QTLs of the BC1 population. Confirmation of QTLs in two successive generations indicates that the QTLs are persistent. The QTLs obtained in this study could be useful in marker-assisted selection of kohlrabi breeding, and to understand the genetic mechanisms of stem swelling and storage organ development in kohlrabi and other Brassica species.

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Acknowledgments

The authors sincerely thank the National Institute of Vegetable and Tea Science, Japan, for kindly providing the DH line, GCP04. This research was partially supported by the Monbukagakusho scholarship of Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT scholarship) for M Hoque. This work was supported by Grant-in-Aid for Challenging Exploratory Research (17 K19262) (JSPS, Japan) to K. Okazaki.

Author contributions: M.H. developed BC1 populations, designed markers, genotype and data collection of BC1 and BC1S1 populations, software running and writing script. D.J.S. provide sequence information for the design of marker and bioinformatics analysis. M.A., M.A.U.D., and M.S. developed the BC1 population from the parents and determined the partial genotype of BC1. M.H., R.F., E.F., and K.O. conceived and designed the experiment. K.O.contributed to the planning, provided funding, and managed the whole project. All authors contributed to the development of this manuscript.

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Authors and Affiliations

  1. Laboratory of Plant Breeding, Graduate School of Science and Technology, Niigata University, 2-8050, Ikarashi, Nishi-ku, Niigata, 950-2181, Japan

    Mozammel Hoque, Daniel J. Shea, Mitsuru Asada, Eigo Fukai & Keiichi Okazaki

  2. Faculty of Agriculture, Sylhet Agricultural University, Sylhet, 3100, Bangladesh

    Mozammel Hoque & Md. Asad-ud-doullah

  3. Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan

    Motoki Shimizu

  4. Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan

    Ryo Fujimoto

  5. Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Nishi-ku, Niigata, 950-2181, Japan

    Keiichi Okazaki

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  1. Mozammel Hoque
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  2. Daniel J. Shea
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Correspondence to Keiichi Okazaki.

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The experiment conducted complies with the laws of Japan.

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Hoque, M., Shea, D.J., Asada, M. et al. QTL map** for tuberous stem formation of kohlrabi (Brassica oleracea var. gongylodes L.). Mol Breeding 37, 109 (2017). https://doi.org/10.1007/s11032-017-0709-6

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