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Improvement of Brassica napus via interspecific hybridization between B. napus and B. oleracea

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Abstract

The large natural variation existing in Brassica oleracea offers a promising approach to improving B. napus (rapeseed). However, the cytogenetic and genetic characterizations of the interspecific hybridization between B. napus and B. oleracea remain poorly understood. Here, the chromosome behavior of F1 triploid hybrids between B. napus and B. oleracea was observed. Various chromosome pairings in pollen mother cells at diakinesis were found with the predominant configuration of 9II + 10I. The segregation pattern of 9:19 had the highest frequency relative to theoretical distribution estimated at anaphase I. Although the fertility was poor in the F1 generation, it recovered to normal levels in only a few generations. Additionally, B. napus-like individuals in the F3 and F4 generations, referred as new-type rapeseed, showed diverse genetic variation relative to current B. napus and strong heterotic potential. Accordingly, a significantly positive correlation between the introgressed B. oleracea genomic components and heterosis was observed in hybrids made with the new-type rapeseed lines. Our data suggest that the introgression of genetic components of B. oleracea can expand the genetic variation and improve the heterotic potential of rapeseed.

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Acknowledgments

This study is partly supported by the Fundamental Research Funds for the Central Universities (XDJK2013A013, XDJK2014C148), the Key Projects in the National Science & Technology (2010BAD01B02), 111 project (B12006), NSFC (31171585), and the open funds of the National Key Laboratory of Crop Genetic Improvement, China.

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Authors and Affiliations

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China

    Qinfei Li, Jiaqin Mei, Yong**g Zhang, Jiana Li & Wei Qian

  2. College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, China

    Qinghong Zhou & Yin**g Huang

  3. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Zaiyun Li & **anhong Ge

  4. Inner Mongolia Potato Engineering and Technology Research Centre, Inner Mongolia University, Hohhot, 010021, China

    Zhiyong **ong

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  1. Qinfei Li
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Correspondence to Wei Qian.

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Qinfei Li and Qinghong Zhou have contributed equally to this work.

Electronic supplementary material

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11032_2014_153_MOESM1_ESM.xlsx

Supplementary materials S1. List of current Brassica napus lines to compare with new type lines for genetic variation revealed with simple sequence repeat markers (XLSX 11 kb)

11032_2014_153_MOESM2_ESM.xlsx

Supplementary materials S2. Distribution of loci amplified with simple sequence repeat primers. The primers were prefixed with BRAS/CB from Celera AgGen Brassica Consortium; SWUC, AG, Pod, YD, Br, h, Bn, CEN from Southwest University; Na, Ra, Ol from BBSRC (Lowe et al. 2003); NIAB from The National Institute of Agricultural Biotechnology; PUT from http://www.plantgdb.org; sR, sN, sS from Agriculture and Agri-Food Canada; BRMS from Suwabe et al. (2002); CNU from Chungnam National University; FITO from http://www.asbornlab.agronomy.wisc.edu/research/maps/ssrs.html; BN were from Szewc-McFadden et al. (1996); MR from Uzanova and Ecke (1999); sOR from Qiu et al.(2006); with ENA and GOL from Choi et al.(2007); CN from Long et al.(2007) (XLSX 17 kb)

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Li, Q., Zhou, Q., Mei, J. et al. Improvement of Brassica napus via interspecific hybridization between B. napus and B. oleracea . Mol Breeding 34, 1955–1963 (2014). https://doi.org/10.1007/s11032-014-0153-9

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