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A Julolidine Coupled Azine-based Reversible Chromo-fluorogenic Probe for Specific Detection of Cu2+ Ions

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Abstract

Among the various essential trace elements for living organisms, the copper (Cu2+) ions are the most important. However, Cu2+ ions are vital for the human body and are associated with necessary physiological processes; insufficient or excessiveness has many hazardous effects on our bodies. In the present contribution, strategically, we have introduced a julolidine-coupled azine-based, 9,9'-((1E,1'E)-hydrazine-1,2-diylidene bis(methanylylidene)) bis(1,2,3,5,6,7-hexahydropyrido [3,2,1-ij] quinolin-8-ol) (HDBQ) reversible chromo-fluorogenic probe for specific detection of Cu2+ ions. Probe HDBQ exhibits observable orange colorimetric change from yellow, which is visible to the naked eye in daylight. The highly green fluorescence HDBQ becomes a non-fluorescent one with the incorporation of Cu2+ ions. Interestingly, the colorimetric change and non-fluorescent HDBQ-Cu2+ complex reverse to the original HDBQ in the presence of ethylenediamine tetraacetic acid (EDTA). The detection and quantification limit of HDBQ towards the detection of Cu2+ ions is found to be in the µM range, which is much lower than the limit (31.5 µM) recommended by WHO. We have also performed a colorimetric and fluorometric paper-based test strips-based experiment employing HDBQ for real-time on-site detection of Cu2+ ions. Using the reversibility characteristics of HDBQ for the consecutive addition of Cu2+ and EDTA, we have established the INHIBIT molecular logic gate. The present report brings a precise and sensitive probe for the detection of Cu2+ ions in real environmental and biological samples.

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Acknowledgements

MNR is highly indebted to DST-FIST, New Delhi, India, for instrumental support. MM and SA are thankful to the government of West Bengal, India, for providing them with the Swami Vivekananda Meritcum-Means Scholarship.

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Authors and Affiliations

  1. Department of Chemistry, University of North Bengal, 734013, Darjeeling, India

    Arpita Maiti, Sabbir Ahamed, Manas Mahato & Mahendra Nath Roy

  2. Department of Chemistry, College, 734001, Siliguri, Darjeeling, India

    Tanusree Ray

  3. Department of Chemistry, Alipurduar University, 736122, Alipurduar, India

    Vikas Kumar Dakua & Kanak Roy

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Authors Arpita Maiti, Sabbir Ahamed and Manas Mahato wrote the main manuscript, prepared figures tables, and conceptualized it. Authors Vikas Kumar Dakua, Kanak Roy and Tanusree Ray reviewed all things.

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Correspondence to Mahendra Nath Roy.

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Maiti, A., Ahamed, S., Mahato, M. et al. A Julolidine Coupled Azine-based Reversible Chromo-fluorogenic Probe for Specific Detection of Cu2+ Ions. J Fluoresc (2024). https://doi.org/10.1007/s10895-023-03577-6

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