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Variation in Preen Oil Composition Pertaining to Season, Sex, and Genotype in the Polymorphic White-Throated Sparrow

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Abstract

Evidence for the the ability of birds to detect olfactory signals is now well documented, yet it remains unclear whether birds secrete chemicals that can be used as social cues. A potential source of chemical cues in birds is the secretion from the uropygial gland, or preen gland, which is thought to waterproof, maintain, and protect feathers from ectoparasites. However, it is possible that preen oil also may be used for individual recognition, mate choice, and signalling social/sexual status. If preen oil secretions can be used as socio-olfactory signals, we should be able to identify the volatile components that could make the secretions more detectable, determine the seasonality of these secretions, and determine whether olfactory signals differ among relevant social groups. We examined the seasonal differences in volatile compounds of the preen oil of captive white-throated sparrows, Zonotrichia albicollis. This species is polymorphic and has genetically determined morphs that occur in both sexes. Mating is almost exclusively disassortative with respect to morph, suggesting strong mate choice. By sampling the preen oil from captive birds in breeding and non-breeding conditions, we identified candidate chemical signals that varied according to season, sex, morph, and species. Linear alcohols with a 10–18 carbon chains, as well as methyl ketones and carboxylic acids, were the most abundant volatile compounds. Both the variety and abundances of some of these compounds were different between the sexes and morphs, with one morph secreting more volatile compounds in the non-breeding season than the other. In addition, 12 compounds were seasonally elevated in amount, and were secreted in high amounts in males. Finally, we found that preen oil signatures tended to be species-specific, with white-throated sparrows differing from the closely related Junco in the abundances and/or prevalence of at least three compounds. Our data suggest roles for preen oil secretions and avian olfaction in both non-social as well as social interactions.

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Acknowledgments

This work was sponsored by the National Institute of Health NIGMS R01GM084229 grant awarded to Elaina M. Tuttle and Rusty Gonser, and by the College of Graduate and Professional Studies at Indiana State University. The Lilly Chemistry Alumni Chair Funds to Milos V. Novotny supported the chemical analyses at the Institute for Pheromone Research, Indiana University. We thank Julie Hagelin for advice and two anonymous reviewers for input. Additional thanks goes to C.A.T. Gonser and D. Sebastian.

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Authors and Affiliations

  1. Department of Biology, Indiana State University, 600 Chestnut Street, Terre Haute, IN, 47809, USA

    Elaina M. Tuttle, Peter J. Sebastian & Rusty A. Gonser

  2. Institute for Pheromone Research and Department of Chemistry, Indiana University, 800 E. Kirkwood Ave., Bloomington, IN, 47405, USA

    Amanda L. Posto, Helena A. Soini & Milos V. Novotny

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  1. Elaina M. Tuttle
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  2. Peter J. Sebastian
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  3. Amanda L. Posto
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  4. Helena A. Soini
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  5. Milos V. Novotny
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  6. Rusty A. Gonser
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Correspondence to Elaina M. Tuttle.

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Tuttle, E.M., Sebastian, P.J., Posto, A.L. et al. Variation in Preen Oil Composition Pertaining to Season, Sex, and Genotype in the Polymorphic White-Throated Sparrow. J Chem Ecol 40, 1025–1038 (2014). https://doi.org/10.1007/s10886-014-0493-2

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