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In vitro antiherpetic activity of compounds extracted from Sargassum fluitans (Børgesen) Børgesen. Identification and in silico evaluation of potential antiviral molecules in a hydroalcoholic extract

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Abstract

Herpes viruses (HSV) are among the most common viruses that infect humans. They cause blisters and inflammation of the oral and genital mucosa as well as severe symptoms such as blindness, hearing impairment and fatal encephalitis. Although acyclovir (ACV) and its derivatives are highly effective in treating recurrent lytic infections, the emergence of resistant strains remains a concern. In this study, the in vitro anti-herpetic activity of a hydroalcoholic extract obtained from the brown seaweed Sargassum fluitans, collected from strandings in Cuba, was evaluated. The hydroalcoholic extract was not cytotoxic in Vero cells (CC50 >5000 µg mL-1) and inhibited replication of HSV-1 (ACV-susceptible and -resistant) and HSV-2 (ACV-resistant) strains with EC50 of 271.66, 403.62 and 14.99 µg mL-1 respectively. In addition, first studies about mechanism of action were evaluated with ACV-sensitive HSV-1 model. The extract showed strong inhibition when pre-incubated with the virus and when the extract and virus were added simultaneously on the cells. The protection of Vero cells during the post-infection assay was very effective up to 1 h. A high resolution UPLC/MS-MS analysis detected 139 compounds, of which 44 were identified. In silico analysis was performed to determine the putative interaction sites of two flavones of interest identified in the extract with the viral glycoprotein D (gD). Molecular docking analysis revealed that takakin and tectorigenin mainly interact with the gD protein active center through hydrophobic interactions (Leu44, Pro50, Thr174, Lys169, Tyr208, Gln209) while hydrogen bonding interactions are less abundant (Ser43, Asp46, Lys169, Tyr208). This research represents a contribution to the valorization of S. fluitans as a source of anti-herpetic compounds in the context of recurrent and abundant seaweed strandings.

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Availability of data and materials

The data that support the findings of this study are available from the corresponding author, [NB].

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Funding

This research was funded by French Embassy Research Scholarship 2021-2022, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université Bretagne Sud (UBS), France and by ISblue project, Interdisciplinary graduate school for the blue planet (ANR-17-EURE-0015) and co-funded by a grant from the French government under the program "Investissements d'Avenir".

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Authors and Affiliations

  1. Department of Microbiology and Virology. Faculty of Biology, University of Havana, 25th Street No. 455 between J and I, Plaza de la Revolución, Havana, Cuba

    Liena de Regla Ponce Rey

  2. Laboratoire de Biotechnologie et Chimie Marines, Université Bretagne Sud, EMR CNRS 6076, IUEM, 56000, Vannes, France

    Hugo Pliego-Cortés, Christel Marty & Nathalie Bourgougnon

  3. Department of Vegetal Biology. Faculty of Biology, University of Havana, 25th Street No. 455 between J and I, Plaza de la Revolución, Havana, Cuba

    Enrique Colina Araujo

  4. Enterovirus National Reference Laboratory, Institute of Tropical Medicine ¨Pedro Kourí¨. Ave. Novia del Mediodía, Km 6 1/2, La Lisa, Havana, Cuba

    Sonia Resik

  5. Marine Research Institute, Loma 35, Alturas del Vedado, Plaza de la Revolución, Havana, Cuba

    Idania Rodeiro Guerra

  6. Platform for the High Resolution Analysis of Biomolecules, La Rochelle Université, UMR CNRS 7266 Littoral Environnement Sociétés LIENSs, Bâtiment ILE, 2rue Olympe de Gouges, 17 000, La Rochelle, France

    Pierre Edouard Bodet

  7. La Rochelle Université, UMR CNRS 7266 Littoral Environnement Sociétés LIENSs, Bâtiment ILE, 2 rue Olympe de Gouges, 17 000, La Rochelle, France

    Laurent Picot

Authors
  1. Liena de Regla Ponce Rey
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  2. Hugo Pliego-Cortés
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  3. Christel Marty
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  4. Enrique Colina Araujo
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  5. Sonia Resik
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  7. Pierre Edouard Bodet
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  8. Laurent Picot
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  9. Nathalie Bourgougnon
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Contributions

Conceptualization: Liena de Regla Ponce Rey, Laurent Picot, Nathalie Bourgougnon

Methodology: Liena de Regla Ponce Rey, Hugo Pliego-Cortés, Christel Marty, Enrique Colina Araujo, Sonia Resik Aguirre, Idania Rodeiro Guerra, Pierre Edouard Bodet, Laurent Picot, Nathalie Bourgougnon

Software: Liena de Regla Ponce Rey, Hugo Pliego-Cortés, Christel Marty, Enrique Colina Araujo, Pierre Edouard Bodet

Validation: Sonia Resik Aguirre, Rodeiro Guerra, Laurent Picot, Nathalie Bourgougnon

Formal analysis: Liena de Regla Ponce Rey

Investigation: Liena de Regla Ponce Rey, Laurent Picot, Nathalie Bourgougnon

Resources: Idania Rodeiro Guerra, Laurent Picot, Nathalie Bourgougnon

Data curation: Liena de Regla Ponce Rey

Writing—original draft preparation: Liena de Regla Ponce Rey

Writing—review and editing: Liena de Regla Ponce Rey, Hugo Pliego-Cortés, Enrique Colina Araujo, Pierre Edouard Bodet, Laurent Picot, Nathalie Bourgougnon

Visualization: Liena de Regla Ponce Rey

Supervision: Laurent Picot, Nathalie Bourgougnon

Project administration: Nathalie Bourgougnon

Funding acquisition: Nathalie Bourgougnon

Corresponding author

Correspondence to Nathalie Bourgougnon.

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Ponce Rey, L., Pliego-Cortés, H., Marty, C. et al. In vitro antiherpetic activity of compounds extracted from Sargassum fluitans (Børgesen) Børgesen. Identification and in silico evaluation of potential antiviral molecules in a hydroalcoholic extract. J Appl Phycol (2024). https://doi.org/10.1007/s10811-024-03202-4

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  • DOI: https://doi.org/10.1007/s10811-024-03202-4

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