Abstract
Phosphoglycerate kinase (PGK), a metabolic enzyme involved in glycolysis and the carbon reduction cycle, is crucial for plant resistance to stresses. However, the role of PGK in highly resilient intertidal seaweeds is unclear. In this study, two PGK genes from the red alga Pyropia haitanensis were isolated by genome-wide screening and PCR cloning. The sequence of cytosolic PhPGK1 was 1355 bp in length, with an open reading frame of 1251 bp, and encodes a protein of 416 amino acids; the sequence of chloroplastic PhPGK2 was 1538 bp in length, with an open reading frame of 1374 bp, and encodes a protein of 457 amino acids. Both PhPGK1 and PhPGK2 contain both conserved structural domains of PGKs and belong to the PGK superfamily. The expression of both PhPGK genes was rapidly induced by high-temperature, and the expression patterns of the two genes were similar, whereas only PhPGK1 was upregulated under high-light stresses. The abiotic stress-tolerance of P. haitanensis thalli was inhibited by PGK inhibitor. Furthermore, ectopic expression of PhPGK1 and PhPGK2 in transgenic Chlamydomonas reinhardtii improved its high-temperature stress tolerance by regulating the expression levels of upstream and downstream genes to synthesize the contents of key metabolites. To our knowledge, this is the first study on the molecular functions of the PGK gene family in seaweed experiencing abiotic stress.
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Acknowledgements
We thank Candace Webb, Ph.D., from Liwen Bianji (Edanz) (www.liwenbianji.cn) for editing the English text of a draft of this manuscript.
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This work was supported by the National Natural Science Foundation of China (grant numbers: U21A20265 and 31872567), the Young Elite Scientists Sponsorship Program by the China Association of Science and Technology (grant number: 2021QNRC001), and the China Agriculture Research System (grant number: CARS-50).
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W.L.W. and C.T.X. conceived of and designed the experiment. Y.Q.L. and W.L.W. performed the experiments and data analysis. C.T.X. and C.S.C. contributed by planning, supervising, and financing the work. D.H.J., K.X., and Y.X. helped to prepare the materials and reagents. Y.Q.L. and W.L.W. drafted and revised the manuscript. All authors read and approved the final manuscript.
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Liao, Y., Ji, D., Xu, Y. et al. Cloning and functional analysis of a phosphoglycerate kinase (PhPGK) from Pyropia haitanensis. J Appl Phycol 35, 1933–1943 (2023). https://doi.org/10.1007/s10811-023-03013-z
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DOI: https://doi.org/10.1007/s10811-023-03013-z