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Immobilized microalgae and bacteria improve salt tolerance of tomato seedlings grown hydroponically

  • 5th Congress of the International Society for Applied Phycology
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Abstract

Wild-type Azospirillum brasilense Cd, mutant Azospirillum brasilense Cd transformed bacteria with a plasmid harboring a trehalose biosynthesis gene fusion from Saccharomyces cerevisiae, and Chlorella vulgaris were used as immobilized inoculants for tomato seedlings growing in hydroponic media prepared with different proportions of NaCl. Stem and main root length, number and length of leaves, and secondary roots of seedlings were measured and compared between treatments as indicator of biomass production. A positive effect in tomato seedlings was observed when co-cultured with Azospirillum (transformed and wild type) and C. vulgaris. Those seedlings growing with wild-type Azospirillum and Chlorella showed longer stems for lower salinities. Conversely, longer stems were observed when transformed Azospirillum and C. vulgaris were used as inoculant in the highest salinity tested (250 mM NaCl).

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Author notes

    Authors and Affiliations

    1. Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Colonia Chamilpa, Cuernavaca, Morelos, 62209, Mexico

      Ramón Suárez

    2. Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 De Febrero 818 Sur, Centro, Cd Obregón, Sonora, 85000, Mexico

      Gilberto Tapia-Reyes

    3. Departamento de Química, Laboratorio de Metabolismo Microbiano, Universidad Autónoma de Guadalajara A.C., Av. Patria 1201, Lomas del Valle, Zapopan, Jalisco, 45110, Mexico

      Froylán M. E. Escalante, Daniel Cortés-Jiménez & Gilberto Tapia-Reyes

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    1. Froylán M. E. Escalante
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    2. Daniel Cortés-Jiménez
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    3. Gilberto Tapia-Reyes
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    4. Ramón Suárez
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    Corresponding author

    Correspondence to Froylán M. E. Escalante.

    Additional information

    Froylán M. E. Escalante and Ramón Suárez contributed equally to this work.

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    Figure 13

    Comparison of the averaged salinity (NaCl) effects in stem length for different inoculants. Columns denoted by a different lower case letter for each salinity level differ significantly at p≤0.05 using a multifactorial ANOVA. Bars represent LSD intervals. (GIF 63 kb)

    High resolution image (TIFF 24680 kb)

    Figure 14

    Comparison of the averaged salinity (NaCl) effects in main root length for different inoculants. Columns denoted by a different lower case letter for each salinity level differ significantly at p≤0.05 using a multifactorial ANOVA. Bars represent LSD intervals. (GIF 68 kb)

    High resolution image (TIFF 24677 kb)

    Figure 15

    Comparison of the averaged salinity (NaCl) effects in secondary roots length for different inoculants. Columns denoted by a different lower case letter for each salinity level differ significantly at p≤0.05 using a multifactorial ANOVA. Bars represent LSD intervals. (GIF 67 kb)

    High resolution image (TIFF 24677 kb)

    Figure 16

    Comparison of the averaged inoculant effects in the number of secondary roots at different salinities, expressed as sodium chloride. Columns denoted by a different lower case letter for each inoculating treatment differ significantly at p≤0.05 using a multifactorial ANOVA. Bars represent LSD intervals. (GIF 60 kb)

    High resolution image (TIFF 24677 kb)

    Figure 17

    Comparison of the averaged salinity (NaCl) effects in the number of secondary roots for different inoculants. Columns denoted by a different lower case letter for each salinity level differ significantly at p≤0.05 using a multifactorial ANOVA. Bars represent LSD intervals. (GIF 60 kb)

    High resolution image (TIFF 24677 kb)

    Figure 18

    Comparison of the averaged salinity (NaCl) effects in leaves length for different inoculants. Columns denoted by a different lower case letter for each salinity level differ significantly at p≤0.05 using a multifactorial ANOVA. Bars represent LSD intervals. (GIF 62 kb)

    High resolution image (TIFF 24677 kb)

    Figure 19

    Averaged number of leaves in seedlings grouped by inoculant used. Columns denoted by a different lower case letter differ significantly at p≤0.05 using a one-way ANOVA. Bars represent LSD intervals. (GIF 14 kb)

    High resolution image (TIFF 24675 kb)

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    Escalante, F.M.E., Cortés-Jiménez, D., Tapia-Reyes, G. et al. Immobilized microalgae and bacteria improve salt tolerance of tomato seedlings grown hydroponically. J Appl Phycol 27, 1923–1933 (2015). https://doi.org/10.1007/s10811-015-0651-0

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