Abstract
Gastric ulcers are caused by an imbalance between aggressive and defensive factors. The green synthesis of silver nanoparticles is becoming a new and promising method in the treatment of gastrointestinal ulcers. This study was conducted to investigate the protective and antioxidant effects of silver nanoparticles synthesized from Quercus brantii extract (NSQBE) on gastric damage induced by alcohol in rats. In this study, silver nanoparticles were produced by the green synthesis method using oak extract. The structure and morphology of nanoparticles were confirmed by various techniques such as UV–Vis spectroscopy, fourier transforms infrared spectrometer (FTIR), scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), energy-dispersive X-ray analysis (EDX), and dynamic light scattering )DLS(. For the animal studies, 30 male Wistar rats weighing 200 ± 20 g were randomly selected and divided into five groups (the normal, ethanolic, NSQBE treatment (received doses of 20 and 5 mg/kg), and standard (received a dose of 50 mg/kg of ranitidine) groups. After the rats were euthanized, their stomach was removed. A part of the stomach tissue of rats was used for histopathological studies, and the other part was used to study biochemical parameters such as the level of reactive oxygen species (ROS), protein carbonyl oxidation (PCO), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and reduced glutathione (GSH) as well as nitric oxide (NO). Our results showed that in the ethanol group, the levels of ROS, MDA, PCO, and serum NO were higher than in the normal group. In addition, reduced GSH, CAT, SOD, tissue NO, gastric mucus, and antioxidant potential were decreased. In rats pretreated with NSQBE and ranitidine, the levels of ROS, MDA, PCO, and serum NO decreased, and the levels of GSH, CAT, SOD, tissue NO, gastric mucus, and antioxidant potential were increased in comparison to the ethanol group. The results of this study showed that silver nanoparticles synthesized using Quercus brantii are a promising approach for the treatment of gastric ulcers.
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References
Aebi H (1984) Catalase in vitro. Methods in enzymology, vol 105. Academic Press, Cambridge, pp 121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
Aghdash HD, Zare-Maivan H, Heydari M, Sharifi M, Lucas-Borja ME, Dey DC (2021) Acorn germination and oak (Quercus brantii Lindl) seedling development were dramatically affected by spatial position of maternal trees from Ilam gas refinery. Iran. Ecologicalengineering 170:106329. https://doi.org/10.1016/j.ecoleng.2021.106329
Al Mofleh IA, Alhaider AA, Mossa JS, Al-Sohaibani MO, Al-Yahya MA, Rafatullah S, Shaik SA (2008) Gastroprotective effect of an aqueous suspension of black cumin Nigella sativa on necrotizing agents-induced gastric injury in experimental animals. Saudi J Gastroenterol 14(3):128. https://doi.org/10.4103/1319-3767.41731
Alrashdi AS, Salama SM, Alkiyumi SS, Abdulla MA, Hadi AH, Abdelwahab SI, Taha MM, Hussiani J, Asykin N (2012) Mechanisms of gastroprotective effects of ethanolic leaf extract of Jasminum sambac against HCl/ethanol-induced gastric mucosal injury in rats. Evid Based Complement Alternat Med 2012:786426. https://doi.org/10.1155/2012/786426
Amaral GP, de Carvalho NR, Barcelos RP, Dobrachinski F, de Lima Portella R, da Silva MH, Boligon AA (2013) Protective action of ethanolic extract of Rosmarinus officinalis L. in gastric ulcer prevention induced by ethanol in rats. Food Chem Toxicol 55:48–55. https://doi.org/10.1016/j.fct.2012.12.038
Arthur I (2001) Introduction-serial review: alcohol, oxidative stress and cell injury. Free Radical Biol Med 31:1524–1526. https://doi.org/10.2141/jpsa.0120090
Azizi S, Pirbalouti AG, Amirmohammadi M (2014) Effect of hydro-alcoholic extract of Persian oak (Quercus brantii) in experimentally gastric ulcer. Iran J Pharma Res 13(3):967
Bafna P, Balaraman R (2004) Anti-ulcer and antioxidant activity of DHC-1, a herbal formulation. J Ethnopharmacol 90(1):123–127. https://doi.org/10.1016/j.jep.2003.09.036
Bahramikia S, Yazdanparast R (2012) EUK-8 and EUK-134 reduce serum glucose and lipids and ameliorate streptozotocin-induced oxidative damage in the pancreas, liver, kidneys, and brain tissues of diabetic rats. Med Chem Res 21:3224–3232. https://doi.org/10.1007/s00044-011-9855-2
Bardi D, Khan MS, Sabri S, Kadir F, Mahmood A, Zahra A, Al-Magrami A (2011) Anti-ulcerogenic activity of Typhonium flagelliforme aqueous leaf extract against ethanol-induced gastric mucosal injury in rats. Sci Res Essays 6(15):3232–3239. https://doi.org/10.5897/SRE11.335
Barmak MJ, Mahmoudi R, Bardania H (2018) Evaluation of the effect of the internal layer of oak fruit (jaft) extract on the prevention of gastric ulcers caused by stress in male rats. J Medicine Life 11(3):225. https://doi.org/10.25122/jml-2017-0025
Behravan M, Panahi AH, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A (2019) Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. Int J Biol Macromol 124:148–154. https://doi.org/10.1016/j.ijbiomac.2018.11.101
Beiranvand M, Bahramikia S (2020) Ameliorating and protective effects mesalazine on ethanol-induced gastric ulcers in experimental rats. Eur J Pharmacol 888:173573. https://doi.org/10.1016/j.ejphar.2020.173573
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239(1):70–76. https://doi.org/10.1006/abio.1996.0292
Brasford M (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Brito SA, de Almeida CLF, de Santana TI, da Silva Oliveira AR, do Nascimento Figueiredo JCB, Souza IT, de Medeiros JW (2018) Antiulcer activity and potential mechanism of action of the leaves of Spondias mombin L. Oxid Med Cell Longev. https://doi.org/10.1155/2018/1731459
Chan FK (2002) Helicobacter pylori, NSAIDs and gastrointestinal haemorrhage. European J Gastroenterol Hepatol 14(1):1–3. https://doi.org/10.1097/00042737-200201000-00001
Chen S, Zhao X, Sun P, Qian J, Shi Y, Wang R (2017) Preventive effect of Gardenia jasminoides on HCl/ethanol induced gastric injury in mice. J Pharmacol Sci 133(1):1–8. https://doi.org/10.1016/j.jphs.2016.05.011
Corne S (1974) A method for the quantitive estimation of gastric barrier mucus. J Physiol (london) 242:116–117. https://doi.org/10.1248/jhs.52.781
Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R (2003) Protein carbonyl groups as biomarkers of oxidative stress. Clin Chim Acta 329(1–2):23–38. https://doi.org/10.1016/S0009-8981(03)00003-2
Das SK, Vasudevan D (2007) Alcohol-induced oxidative stress. Life Sci 81(3):177–187. https://doi.org/10.1016/j.lfs.2007.05.005Get
Dashputre N, Naikwade N (2011) Evaluation of anti-ulcer activity of methanolic extract of Abutilon indicum Linn leaves in experimental rats. Int J Pharm Sci Drug Res 3(2):97–100
De M, De Krishna A, Banerjee A (1999) Antimicrobial screening of some Indian spices. Phytother Res 13(7):616–618. https://doi.org/10.1002/(SICI)1099-1573(199911)13:7%3c616::AID-PTR475%3e3.0.CO;2-V
Faramarzian M, Bahramikia S (2020) The effects of Quercus brantii acorn extract on hen egg-white lysozyme amyloid formation and disassemble amyloid aggregates. J Food Process Preserv 44(7):e14499. https://doi.org/10.1111/jfpp.14499
Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59(2):309–314. https://doi.org/10.1104/pp.59.2.309
Jamshidi A, Rezaei S, Hassani G, Firoozi Z, Ghaffari HR, Sadeghi H (2020) Coagulating potential of Iranian oak (Quercus Branti) extract as a natural coagulant in turbidity removal from water. J Environ Health Sci Eng 18:163–175. https://doi.org/10.1007/s40201-020-00449-0
Jollow D, Mitchell J, Zampaglione NA, Gillette J (1974) Bromobenzene-induced liver necrosis Protective role of glutathione and evidence for 3, 4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11(3):151–169. https://doi.org/10.1159/000136485
Kahraman A, Erkasap N, Köken T, Serteser M, Aktepe F, Erkasap S (2003) The antioxidative and antihistaminic properties of quercetin in ethanol-induced gastric lesions. Toxicology 183(1–3):133–142. https://doi.org/10.1016/S0300-483X(02)00514-0
Kaji I, Kaunitz JD (2017) Luminal chemosensing in the gastroduodenal mucosa. Currentopinioningastroenterology 33(6):439. https://doi.org/10.1097/MOG.0000000000000396
Kavitt RT, Lipowska AM, Anyane-Yeboa A, Gralnek IM (2019) Diagnosis and treatment of peptic ulcer disease. Am J Med 132(4):447–456. https://doi.org/10.1097/MOG.0000000000000396
Khosravi AD, Behzadi A (2006) Evaluation of the antibacterial activity of the seed hull of Quercus brantii on some gram negative bacteria. Pak J Med Sci 22(4):429–432
Kim J-H, Choi S-K, Choi S-Y, Kim H-K, Chang H-I (2005) Suppressive effect of astaxanthin isolated from the Xanthophyllomyces dendrorhous mutant on ethanol-induced gastric mucosal injury in rats. Biosci Biotechnol Biochem 69(7):1300–1305. https://doi.org/10.1271/bbb.69.1300
Liu B, Feng X, Zhang J, Wei Y, Zhao X (2019) Preventive effect of Anji white tea flavonoids on alcohol-induced gastric injury through their antioxidant effects in Kunming mice. Biomolecules 9(4):137. https://doi.org/10.3390/biom9040137
Mohanpuria P, Rana NK, Yadav SK (2008) Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res 10:507–517. https://doi.org/10.1007/s11051-007-9275-x
Musumba C, Pritchard D, Pirmohamed M (2009) cellular and molecular mechanisms of NSAID-induced peptic ulcers. Aliment Pharmacol Ther 30(6):517–531. https://doi.org/10.1111/j.1365-2036.2009.04086.x
Pathak J, Chatterjee A, Singh SP, Sinha RP (2017) Detection of reactive oxygen species (ROS) in cyanobacteria using the oxidant-sensing probe 2’, 7’-dichlorodihydrofluorescein diacetate (DCFH-DA). Bio-Protoc 7(17):e2545. https://doi.org/10.21769/BioProtoc.2545
Packer PL, Halliwell BL (1992) Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation. Biochem J 286:607–611
Reddy G, Gandhi N (2012) Environmental friendly biosynthesis, characterization and antibacterial activity of silver nanoparticles by using Senna Saimea plant leaf aqueous extract. Int J Iins Pharm Life Sci 2(1):186–193
Salem NA, Wahba MA, Eisa WH, El-Shamarka M, Khalil W (2018) Silver oxide nanoparticles alleviate indomethacin-induced gastric injury: a novel antiulcer agent. Inflammopharmacology 26(4):1025–1035. https://doi.org/10.1007/s10787-017-0424-2
Samuei A, Divya S, Sindu S, Arumugam P (2014) Studies on synthesis, characterization and application of silver nano-particles using mimosa pudica leaves. Int J Pharm Pharm Sci 2:453–455
Sathish R, Sahu A, Natarajan K (2011) Antiulcer and antioxidant activity of ethanolic extract of Passiflora foetida L. Indian J Pharmacol 43(3):336–339
Sheu S-Y, Tsuang Y-H, Hsu F-L, Lu F-J, Chiang H-C (1997) Superoxide anion scavenge effect of quercs glauca thunb in whole blood of patients with ankylosing spondylitis. Am J Chinese Med 25(03n04):307–315. https://doi.org/10.1142/S0192415X97000342
Sidahmed HMA, Azizan AHS, Mohan S, Abdulla MA, Abdelwahab SI, Taha MME, Loke MF (2013) Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity. BMC Complement Altern Med 13:1–15. https://doi.org/10.1186/1472-6882-13-183
Tamura M, Matsui H, Kaneko T, Hyodo I (2013) Alcohol is an oxidative stressor for gastric epithelial cells: detection of superoxide in living cells. J Clin Biochem Nutr 53(2):75–80. https://doi.org/10.3164/jcbn.13-32
Thangaraju N, Venkatalakshmi R, Chinnasamy A, Kannaiyan P (2012) Synthesis of silver nanoparticles and the antibacterial and anticancer activities of the crude extract of Sargassum polycystum C. Agardh Nano Biomed Eng 4(2):89–94. https://doi.org/10.5101/nbe.v4i2.p89-94
Veisi H, Hemmati S, Shirvani H, Veisi H (2016) Green synthesis and characterization of monodispersed silver nanoparticles obtained using oak fruit bark extract and their antibacterial activity. Appl Organomet Chem 30(6):387–391. https://doi.org/10.1002/aoc.3444
Velhal S, Latpate R, Kulkarni S, Jaybhaye R (2015) Taguchi design for parameter optimization of size-controlled synthesis of silver nanoparticles. Int J Emerg Technol Comput Appl Sci 12:144–149. https://doi.org/10.17485/IJST/v14i38.1065
Yoro M, Garba A, Joshua J, Ayuba I (2022) Green Synthesis, characterization and antimicrobial potency of silver nanoparticles from psidium guajava leaf extract. J Chem. https://doi.org/10.31586/ojc.2022.255
Zeb A, Ullah F (2016) A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods. J Anal Methods Chem. https://doi.org/10.1155/2016/9412767
Zeng Q, Jiang X, Yu A, Lu GM (2007) Growth mechanisms of silver nanoparticles: a molecular dynamics study. Nanotechnology 18(3):035708. https://doi.org/10.1088/0957-4484/18/3/035708
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This study was supported by a grant from the Faculty of Basic Sciences, Lorestan University. The founding sponsor had no role in the study design, performance, data collection, and analysis, decision to publish, or preparation/writing of the manuscript.
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SB: participates in the design and interpretation of the studies and the review of the manuscript. SS: conducted the experiments and wrote the manuscript, and all images were drawn. OD: participated in the preparation of tissue samples and pathological analysis. All authors’ approval for publication.
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Safari, S., Bahramikia, S. & Dezfoulian, O. Silver nanoparticles synthesized from Quercus brantii ameliorated ethanol-induced gastric ulcers in rats by decreasing oxidative stress and improving antioxidant systems. Inflammopharmacol 31, 2615–2630 (2023). https://doi.org/10.1007/s10787-023-01284-z
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DOI: https://doi.org/10.1007/s10787-023-01284-z