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Effect of Psoralen on the Intestinal Barrier and Alveolar Bone Loss in Rats With Chronic Periodontitis

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Abstract

To study the effects of psoralen on the intestinal barrier and alveolar bone loss (ABL) in rats with chronic periodontitis. Fifty-two 8-week-old specific pathogen-free (SPF) male Sprague-Dawley (SD) rats were randomly divided into the following four groups: Control group (Control), psoralen group of healthy rats (Pso), periodontitis model group (Model), and psoralen group of periodontitis rats (Peri+Pso). The alveolar bone resorption of maxillary molars was observed via haematoxylin-eosin staining and micro-computed tomography. The expression level of receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG) in periodontal tissues was evaluated by immunofluorescence staining. The changes in serum tumour necrosis factor (TNF)-α, interleukin (IL)-10, IL-6, intestinal mucosal occludin, and claudin-5 were detected using enzyme-linked immunosorbent assay (ELISA). The level of intestinal mucosal NOD2 was detected using immunohistochemical methods. DNA was extracted from the intestinal contents and the 16s rRNA gene was sequenced using an Illumina MiSeq platform. The expression of NOD2 protein in the intestinal tract of periodontitis rats decreased after intragastric psoralen administration. Psoralen increased the intestinal microbiota diversity of rats. The level of serum pro-inflammatory factor TNF-α decreased and the level of anti-inflammatory factor IL-10 increased. ABL was observed to be significantly decreased in rats treated with psoralen. Psoralen decreased the RANKL/OPG ratio of periodontitis rats. Psoralen may affect the intestinal immune barrier and ecological barrier, mediate immune response, promote the secretion of anti-inflammatory factor IL-10, and reduce the secretion of the pro-inflammatory factor TNF-α, thus reducing ABL in experimental periodontitis in rats.

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Funding

This work was supported by the Shandong Province 2019-2020 TCM Technology Development Plan [No. 2019-0618] and the Qingdao Key Health Discipline Development Fund [No. 2020].

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  1. Hua Liu and Yingjie Xu contributed equally to this work.

Authors and Affiliations

  1. Department of Stomatology, School of Stomatology of Qingdao University, Qingdao, 266003, Shandong Province, China

    Hua Liu

  2. Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong Province, China

    Yingjie Xu, Qi Cui, Ning Liu, Fuhang Chu, Beibei Cong & Yingtao Wu

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Contributions

Hua Liu and Yingjie Xu conceived and designed the study. Hua Liu, Yingjie Xu, Qi Cui, Ning Liu, Fuhang Chu, Beibei Cong, and Yingtao Wu performed the experiments. Hua Liu and Yingjie Xu wrote the paper. All authors read and approved the manuscript.

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Correspondence to Yingtao Wu.

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Liu, H., Xu, Y., Cui, Q. et al. Effect of Psoralen on the Intestinal Barrier and Alveolar Bone Loss in Rats With Chronic Periodontitis. Inflammation 44, 1843–1855 (2021). https://doi.org/10.1007/s10753-021-01462-7

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