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Obesity Exacerbates Sepsis-Induced Oxidative Damage in Organs

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Abstract

Sepsis progression is linked to the imbalance between reactive oxygen species and antioxidant enzymes. Sepsis affects multiple organs, but when associated with a chronic inflammatory disease, such as obesity, it may be exacerbated. We hypothesized that obesity could aggravate the oxidative damage to peripheral organs of rats submitted to an animal model of sepsis. Male Wistar rats aged 8 weeks received hypercaloric nutrition for 2 months to induce obesity. Sepsis was induced by cecal ligation and puncture (CLP) procedure, and sham-operated rats were considered as control group. The experimental groups were divided into sham + eutrophic, sham + obese, CLP + eutrophic, and CLP + obese. Twelve and 24 h after surgery, oxidative damage to lipids and proteins and superoxide dismutase (SOD) and catalase (CAT) activities were evaluated in the liver, lung, kidney, and heart. The data indicate that obese rats subjected to sepsis present oxidative stress mainly in the lung and liver. This alteration reflected an oxidative damage to lipids and proteins and an imbalance of SOD and CAT levels, especially 24 h after sepsis. It follows that obesity due to its pro-inflammatory phenotype can aggravate sepsis-induced damage in peripheral organs.

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Acknowledgments

This research was supported by the Programa de Pós-graduação em Ciências da Saúde–UNISUL and the CNPq.

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Authors and Affiliations

  1. Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil

    Fabricia Petronilho, Amanda Della Giustina, Diego Zapelini Nascimento, Graciela Freitas Zarbato, Andriele Aparecida Vieira, Drielly Florentino, Lucinéia Gainski Danielski, Mariana Pereira Goldim & Gislaine Tezza Rezin

  2. Center for Experimental Models in Psychiatry, Department of Psychiatry and Behavioral Sciences, The University of Texas Medical School at Houston, Houston, TX, USA

    Tatiana Barichello

  3. Laboratory of Experimental Microbiology, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, SC, Brazil

    Tatiana Barichello

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  1. Fabricia Petronilho
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Correspondence to Fabricia Petronilho.

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All studies were performed in compliance with the National Institutes of Health Guidelines and with the approval of the Animal Care and Experimentation Committee of UNISUL (protocol number 13.026.4.03.IV), Brazil.

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Petronilho, F., Giustina, A.D., Nascimento, D.Z. et al. Obesity Exacerbates Sepsis-Induced Oxidative Damage in Organs. Inflammation 39, 2062–2071 (2016). https://doi.org/10.1007/s10753-016-0444-x

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