Abstract
In this work, the anti-asthma potential of platycodin D (PLD) was studied by investigation of its effect to suppress airway inflammation, a murine model of asthma and the possible mechanisms. A total of 50 mice were randomly assigned to five experimental groups: control, ovalbumin (OVA), OVA+ dexamethasone (2 mg/kg) and OVA + PLD (40, 80 mg/kg). Airway resistance (Raw) were measured; airway histological studies were evaluated by the hematoxylin and eosin (HE) staining; interleukin-4 (IL-4), interleukin-5(IL-5), and interleukin-13 in bronchoalveolar lavage fluid (BALF) were evaluated by enzyme-linked immunosorbent assay (ELISA); NF-κBp65, p-NF-κBp65, p-IKKα, IKKα, p-IKKβ, p-IкBα, and IкBα of airway were measured by Western blotting. Our study demonstrated that PLD inhibited OVA-induced increases in Raw and eosinophil count in airway; IL-4, IL-5, and IL-13 were recovered in BALF. Histological studies demonstrated that PLD substantially inhibited OVA-induced eosinophilia in airway tissue. Western blotting studies demonstrated that PLD substantially inhibited NF-κB pathway. These findings suggest that PLD may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beasley, R., J. Crane, C.K. Lai, and N. Pearce. 2000. Prevalence and etiology of asthma. Journal of Allergy and Clinical Immunology 105: 466–72.
Busse, W.W., and R.F. Lemanske Jr. 2001. Asthma. New England Journal of Medicine 344: 350–62.
Brightling, C.E., F.A. Symon, S.S. Birring, et al. 2002. TH2 cytokine expression in bronchoalveolar lavage fluid T lymphocytes and bronchial submucosa is a feature of asthma and eosinophilic bronchitis. Journal of Allergy and Clinical Immunology 110: 899–905.
Robinson, D.S., Q. Hamid, S. Ying, et al. 1992. Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma. New England Journal of Medicine 326: 298–304.
Hershey, G.K. 2003. IL-13 receptors and signaling pathways: an evolving web. Journal of Allergy and Clinical Immunology 111: 677–690.
Ci, X., X. Chu, X. Xu, H. Li, and X. Deng. 2012. Short-term roxithromycin treatment attenuates airway inflammation via MAPK/NF-kappaB activation in a mouse model of allergic asthma. Inflammation Research 61: 749–58. 7. L.-K.
Han, B., J. Xu, and Y. Kimura. 2000. Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity. Journal of Nutrition 130(11): 2760–2764.
Han, L.-K., Y.-N. Zheng, B.-J. Xu, H. Okuda, and Y. Kimura. 2002. Saponins from Platycodi radix ameliorate high fat diet-induced obesity in mice. Journal of Nutrition 132(8): 2241–2245.
Kim, Y.P., E.B. Lee, and S.Y. Kim. 2001. Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum. Planta Medica 67(4): 362–364.
Kim, M.-O., D.-O. Moon, H.C. Yung, J.-D. Lee, D.K. Nam, and G.-Y. Kim. 2008. Platycodin D induces mitotic arrest in vitro, leading to endoreduplication, inhibition of proliferation and apoptosis in leukemia cells. International Journal of Cancer 122(12): 2674–2681.
Choi, S.-S., E.-J. Han, T.-H. Lee, et al. 2002. Antinociceptive mechanisms of platycodin D administered intracerebroventricularly in the mouse. Planta Medica 68(9): 794–798.
**e, Y., H.-X. Sun, and D. Li. 2009. Platycodin D is a potent adjuvant of specific cellular and humoral immune responses against recombinant hepatitis B antigen. Vaccine 27(5): 757–764.
Oh, S.R., M.Y. Lee, K. Ahn, B.Y. Park, O.K. Kwon, H. Joung, J. Lee, D.Y. Kim, S. Lee, J.H. Kim, and H.K. Lee. 2006. Suppressive effect of verproside isolated from Pseudolysimachion longifolium on airway inflammation in a mouse model of allergic asthma. International Immunopharmacology 6: 978–86.
Djukanović, R., W.R. Roche, J.W. Wilson, C.R. Beasley, O.P. Twentyman, R.H. Howarth, and S.T. Holgate. 1990. Mucosal inflammation in asthma. American Journal of Respiratory and Cirtical Care Medicine 142: 434–57.
Duan, W., J.H. Chan, C.H. Wong, B.P. Leung, and W.S. Wong. 2004. Anti inflammatory effects of mitogen-activated protein kinase inhibitor U0126 in an asthma mouse model. The Journal of Immunology 172: 7053–7059.
Wenzel, S.E. 2006. Asthma: defining of the persistent adult phenotypes. Lancet 368: 804–13.
Elsner, J., and A. Kapp. 1999. Regulation and modulation of eosinophil effector functions. Allergy 54: 15–22.
Haddad, J.J. 2002. Nuclear factor (NF)-kappa B blockade attenuates but does not abrogate LPS-mediated interleukin (IL)-1 beta biosynthesis in alveolar epithelial cells. Biochemical and Biophysical Research Communications 293: 252–257.
Haeberle, H.A., R. Takizawa, A. Casola, A.R. Brasier, H.J. Dieterich, N. Van Rooijen, et al. 2002. Respiratory syncytial virus-induced activation of nuclear factor-kappaB in the lung involves alveolar macrophages and toll-like receptor 4-dependent pathways. Journal of Infectious Diseases 186: 1199–1206.
Poynter, M.E., C.G. Irvin, and Y.M. Janssen-Heininger. 2002. Rapid activation of nuclear factor-kappaB in airway epithelium in a murine model of allergic airway inflammation. American Journal of Pathology 160: 1325–1334.
Yang, L., L. Cohn, D.H. Zhang, R. Homer, A. Ray, and P. Ray. 1998. Essential role of nuclear factor kappaB in the induction of eosinophilia in allergic airway inflammation. Journal of Experimental Medicine 188: 1739–1750.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Zhang, T., Yang, S., Du, J. et al. Platycodin D Attenuates Airway Inflammation in a Mouse Model of Allergic Asthma by Regulation NF-κB Pathway. Inflammation 38, 1221–1228 (2015). https://doi.org/10.1007/s10753-014-0089-6
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10753-014-0089-6