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An acute increase in water temperature can increase free amino acid concentrations in the blood, brain, liver, and muscle in goldfish (Carassius auratus)

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Abstract

Water temperature directly affects the body temperature in fish, so increasing water temperatures in oceans and rivers will lead to increases in fish body temperatures. Whilst a range of responses of fish to increases in water temperature have been measured, amino acid metabolism in a fish under high water temperature (HT) conditions has not been investigated. The aim of this study was to determine the effects of an acute increase in water temperature on oxygen consumption, plasma cortisol concentrations, and free amino acid concentrations in plasma and several tissues in goldfish (Carassius auratus). Oxygen consumption and plasma cortisol concentrations were increased in goldfish exposed to HT (30 ± 1 °C) for 200 min compared with goldfish at a control water temperature (CT 17 ± 1 °C). Oxygen consumption and plasma cortisol concentrations in both groups of fish combined were positively correlated. When goldfish were exposed to HT for 300 min oxygen consumption and plasma concentrations of 15 free amino acids were increased compared with goldish at CT. Concentrations of several free amino acids were increased to varying extents in the brain, liver, and muscle tissues. In conclusion, an acute increase in water temperature affected amino acid metabolism differently in the brain, liver, and muscle tissues. Goldfish will be a useful species for further studies of the possible roles of various amino acids in the brain, muscle, and liver during acute increases in water temperature in fish.

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Acknowledgments

The diligent technical support of Shin Oikawa (Retired Associate Professor, Fishery Research Laboratory, Kyushu University) in the measurement of oxygen consumption in goldfish is gratefully acknowledged.

Funding

This study was supported by JSPS KAKENHI grant numbers JP15K07694 and JP18K19271 to VSC.

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Authors and Affiliations

  1. Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan

    Yunhao Wang, Guofeng Han, Cuong V. Pham & Mitsuhiro Furuse

  2. Laboratory of Stress Physiology and Metabolism, Faculty of Arts and Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 819-0395, Japan

    Yunhao Wang, Guofeng Han, Cuong V. Pham & Vishwajit S. Chowdhury

  3. Fishery Research Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan

    Kiyohiko Koyanagi

  4. Laboratory of Cognative Neuroscience, Graduate School of System Life Sciences, Kyushu University, Fukuoka, 819-0395, Japan

    Yandejia Song, Ryunosuke Sudo & Johan Lauwereyns

  5. School of Veterinary Science, Massey University, Palmerston North, 4442, New Zealand

    John F. Cockrem

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  1. Yunhao Wang
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  2. Guofeng Han
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  9. Mitsuhiro Furuse
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  10. Vishwajit S. Chowdhury
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Correspondence to Vishwajit S. Chowdhury.

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This study was performed in accordance with the ethical guidelines for animal experiments as described in the materials and methods section.

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Wang, Y., Han, G., Pham, C.V. et al. An acute increase in water temperature can increase free amino acid concentrations in the blood, brain, liver, and muscle in goldfish (Carassius auratus). Fish Physiol Biochem 45, 1343–1354 (2019). https://doi.org/10.1007/s10695-019-00642-5

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