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Evaluation and genome-wide association study of Verticillium wilt resistance in a MAGIC population derived from intermating of eleven Upland cotton (Gossypium hirsutum) parents

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Abstract

Vertcillium wilt (VW, Verticillium dahliae Kleb.) is one of the most important soil-borne fungal diseases affecting world cotton production. Numerous studies have been conducted to investigate the genetic basis of VW resistance using bi-parental or association map** populations. In this study, a multi-parent advanced generation inter-cross (MAGIC) population of 550 recombinant inbred lines (RILs) together with their 11 Upland cotton parents with a total of 473,516 polymorphic SNP markers was used to identify chromosomal regions for VW resistance based on foliar disease severity ratings (DSR) in two replicated greenhouse tests. Among the 11 parents, Acala Ultima, Coker 315, and Deltapine Acala 90 in the two tests and FiberMax 966 in one test and on average were significantly more resistant than the susceptible check parent- Phytogen PSC 355, while Paymaster HS 26, SureGrow 747, Stoneville 474, Stoneville 825, and Tamcot Pyramid performed similarly to PSC 355. Although a significant genotype × test interaction was detected for DSR, 37 MAGIC lines were consistently resistant to VW. Of 13 and 12 VW resistance QTL declared in Test 1 and 2, respectively, through a genome-wide association study (GWAS), three QTL on chromosome A01, D02 and D08 were in common and three QTL clusters were detected on chromosome A01, A13, and D01 between the two tests. The narrow chromosomal regions for the three common resistance QTL allowed identification of possible candidate genes for VW resistance. This greenhouse study identified VW resistant parents and RILs and common resistance QTL for VW resistance, providing useful information for develo** VW resistant cultivars and further genomic studies towards the identification of VW resistance genes in cotton.

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Author notes

  1. **fa Zhang, Abdelraheem Abdelraheem and Gregory N. Thyssen have contributed equally to this work.

Authors and Affiliations

  1. Department of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM, 88003, USA

    **fa Zhang & Abdelraheem Abdelraheem

  2. Cotton Fiber Bioscience & Cotton Chemistry and Utilization Research Units, USDA-ARS-SRRC, New Orleans, LA, USA

    Gregory N. Thyssen

  3. Cotton Fiber Bioscience and Research Units, USDA-ARS-SRRC, New Orleans, LA, USA

    David D. Fang

  4. Crop Science Research Laboratory, USDA-ARS, Mississippi State, MS, USA

    Johnie N. Jenkins & Jack C. McCarty Jr

  5. Cotton Incorporated, Cary, NC, USA

    Tom Wedegaertner

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  1. **fa Zhang
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Correspondence to **fa Zhang.

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Zhang, J., Abdelraheem, A., Thyssen, G.N. et al. Evaluation and genome-wide association study of Verticillium wilt resistance in a MAGIC population derived from intermating of eleven Upland cotton (Gossypium hirsutum) parents. Euphytica 216, 9 (2020). https://doi.org/10.1007/s10681-019-2547-6

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