Abstract
To investigate the removal of organochlorine pesticide residues by immobilized degrading microbe, indigenous microorganisms from organochlorine pesticide (OCP)–contaminated soils in Chengdu plain, pot experiments were carried out to evaluate the potential of the immobilized complex microbial specific degrading microbe treated with sodium alginate (SA) composite carrier in decontaminating OCP-contaminated soils, and field experiments were also conducted to investigate the enhanced efficiency of immobilized microbial agents on the dissipation of OCPs in the contaminated plots for different cultivation usage. The results showed that the dissipation rate of OCPs in contaminated soils with initial concentrations of 122.24 μg/kg was 89.94% after the addition of 25 mg of immobilized microbial agents at the end of the 90 days of experiment, which was 6.1% higher than that of the compound microbial agents under the same environmental conditions, and the control group without the addition of microbial agents was only 1.18%, while the concentration of OCPs in contaminated soils with initial concentrations of 203.64 μg/kg only decreased to 65.29 μg/kg after the addition of 20 mg of compound microbial agents. In contrast, the soil concentration of immobilized microbial agent treatment group under the same conditions decreased to 52.15 μg/kg. During the field experiment, the enhanced efficiency of immobilized microbial agents on the degradation of OCPs in different cultivation usage was evidently different, showed that the concentration of OCPs in paddy fields (18.60%) > tea gardens (12.17%) ≥ orchards (11.41%) > vegetable fields (6.21%) ≥ dryland (4.79%), which was especially significant in stress environment. Overall, the immobilization treatment obviously improved the degradation potential of OCPs-specific degrading microbe, and the degree of improvement was related to the metabolic activity of the degrading microbe, the addition amount, remediation time, and habitat conditions.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
References
Chen, X. Y., He, B., Feng, M., Zhao, D. W., & Sun, J. (2020). Immobilized laccase on magnetic nanoparticles for enhanced lignin model compounds degradation. Chinese Journal of Chemical Engineering, 28, 2152–2159.
Covarrubias, S. A., & de-Bashan LE, Moreno M, Bashan Y,. (2012). Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae. Applied Microbiology and Biotechnology, 93, 2669–2680.
Deng, F. C., Liao, C. J., Yang, C., Guo, C. L., & Dang, Z. (2016). Enhanced biodegradation of pyrene by immobilized bacteria on modified biomass materials. International Biodeterioration and Biodegradation, 110, 46–52.
Derakhshan Nejad, Z., Jung, M. C., & Kim, K. H. (2018). Remediation of soils contaminated with heavy metals with an emphasis on immobilization technology. Environmental Geochemistry and Health, 40, 927–953.
Gao, H. J., Jiang, X., Wang, F., Wang, D. Z., & Bian, Y. R. (2005). Residual levels and bioaccumulation of chlorinated persistent organic pollutants (POPs) in vegetables from suburb of Nan**g, People’s Republic of China. Bulletin of Environmental Contamination and Toxicology, 74, 673–680.
Hu, G. C., Dai, J. Y., Mai, B. X., Luo, X. J., Cao, H., Wang, J. S., Li, F. C., & Xu, M. Q. (2010). Concentrations and accumulation features of organochlorine pesticides in the Baiyangdian Lake freshwater food web of North China. Archives of Environmental Contamination and Toxicology, 58, 700–710.
Jiang, Y., Yang, F., Dai, M., Ali, I., Shen, X., Hou, X., Alhewairini, S. S., Peng, C., & Naz, I. (2022). Application of microbial immobilization technology for remediation of Cr(VI) contamination: A review. Chemosphere, 286, 131721.
Jiang, Y. F., Wang, X. T., Jia, Y., Wang, F., Wu, M. H., Sheng, G. Y., & Fu, J. M. (2009). Occurrence, distribution and possible sources of organochlorine pesticides in agricultural soil of Shanghai, China. Journal of Hazardous Materials, 170, 989–997.
Kamanavalli, C. M., & Ninnekar, H. Z. (2004). Biodegradation of DDT by a Pseudomonas species. Current Microbiology, 48, 10–13.
Khalid, S., Shahid, M., Niazi, N. K., Murtaza, B., Bibi, I., & Dumat, C. (2017). A comparison of technologies for remediation of heavy metal contaminated soils. Journal of Geochemical Exploration, 182, 247–268.
Landrigan, P. J., et al. (2020). Human health and ocean pollution. Annals of Global Health, 86, 151.
Li, M. Y., Zhang, X. T., Liu, H. Y., & Wei, S. Q. (2022). Effects of water management on the transformation of iron oxide forms in paddy soils and its coupling with changes in cadmium activity. Huan **g Ke Xue = Huan**g Kexue, 43, 4301–4312.
Li, Y., Niu, J., Shen, Z., Zhang, C., Wang, Z., & He, T. (2014). Spatial and seasonal distribution of organochlorine pesticides in the sediments of the Yangtze Estuary. Chemosphere, 114, 233–240.
Ma, L., Hu, T., Liu, Y., Liu, J., Wang, Y., Wang, P., Zhou, J., Chen, M., Yang, B., & Li, L. (2021). Combination of biochar and immobilized bacteria accelerates polyacrylamide biodegradation in soil by both bio-augmentation and bio-stimulation strategies. Journal of Hazardous Materials, 405, 124086.
Magrí, A., Vanotti, M. B., & Szögi, A. A. (2012). Anammox sludge immobilized in polyvinyl alcohol (PVA) cryogel carriers. Bioresource Technology, 114, 231–240.
Melkonian, C., Fillinger, L., Atashgahi, S., da Rocha, U. N., Kuiper, E., Olivier, B., Braster, M., Gottstein, W., Helmus, R., Parsons, J. R., Smidt, H., van der Waals, M., Gerritse, J., Brandt, B. W., Röling, W. F. M., Molenaar, D., & van Spanning, R. J. M. (2021). High biodiversity in a benzene-degrading nitrate-reducing culture is sustained by a few primary consumers. Communications Biology, 4, 530.
Nagata, Y., Nariya, T., Ohtomo, R., Fukuda, M., Yano, K., & Takagi, M. (1993). Cloning and sequencing of a dehalogenase gene encoding an enzyme with hydrolase activity involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis. Journal of Bacteriology, 175, 6403–6410.
Ndunda, E. N., Madadi, V. O., & Wandiga, S. O. (2018). Organochlorine pesticide residues in sediment and water from Nairobi River, Kenya: Levels, distribution, and ecological risk assessment. Environmental Science and Pollution Research, 25, 34510–34518.
Nzila, A., Musa, M. M., Sankara, S., Al-Momani, M., **ang, L., & Li, Q. X. (2021). Degradation of benzo[a]pyrene by halophilic bacterial strain Staphylococcus haemoliticus strain 10SBZ1A. PLoS ONE, 16, e0247723.
Pan, T., Huang, P., **ong, G., & Maser, E. (2015). Isolation and identification of a repressor TetR for 3,17β-HSD expressional regulation in Comamonas testosteroni. Chemico-Biological Interactions, 234, 205–212.
Schwarzbauer, J., Ricking, M., & Littke, R. (2003). DDT-related compounds bound to the nonextractable particulate matter in sediments of the Teltow Canal, Germany. Environmental Science and Technology, 37, 488–495.
Selvam, K., Govarthanan, M., Senbagam, D., Kamala-Kannan, S., Senthilkumar, B., & Selvankumar, T. (2016). Activity and stability of bacterial cellulase immobilized on magnetic nanoparticles. Chinese Journal of Catalysis, 37, 1891–1898.
Simarro, R., Gonzalez, N., Bautista, L. F., & Molina, M. C. (2013). Assessment of the efficiency of in situ bioremediation techniques in a creosote polluted soil: Change in bacterial community. Journal of Hazardous Materials, 262, 158–167.
Singh, T., & Singh, D. K. (2019). Rhizospheric microbacterium sp. P27 showing potential of lindane degradation and plant growth promoting traits. Current Microbiology, 76, 888–895.
Su, D., Li, P. J., Wang, X., & Xu, H. X. (2007). Degradation and kinetics of pyrene and benzo [a] pyrene by three bacteria in contaminated soil. Huan **g Ke Xue = Huan**g Kexue, 28, 913–7.
Tran, D. M., Huynh, T. U., Nguyen, T. H., Do, T. O., Nguyen, Q. V., & Nguyen, A. D. (2022). Molecular analysis of genes involved in chitin degradation from the chitinolytic bacterium Bacillus velezensis. Antonie van Leeuwenhoek, 115, 215–231.
Tudi, M., Ruan, D. H., Wang, L., Lyu, J., Sadler, R., Connell, D., Chu, C., & Phung, D. T. (2021). Agriculture development, pesticide application and its impact on the environment. International Journal of Environmental Research and Public Health, 18.
Velkova, Z., Kirova, G., Stoytcheva, M., Kostadinova, S., Todorova, K., & Gochev, V. (2018). Immobilized microbial biosorbents for heavy metals removal. Engineering in Life Sciences, 18, 871–881.
Wang, X., Li, P., Gong, Z., Li, B., Ju, J., He, X., & Tai, P. (2001). Degradation of phenanthrene and pyrene in contaminated soil by immobilized Zoogloea sp. and Fusarium sp. Ying Yong Sheng Tai Xue Bao = The Journal of Applied Ecology, 12(4), 636–638.
Wang, X., Li, P., Song, S., Zhong, Y., Zhang, H., & Verkhozina, E. V. (2006). Immobilization of introduced bacteria and degradation of pyrene and benzo(alpha) pyrene in soil by immobilized bacteria. Ying Yong Sheng Tai Xue Bao = The Journal of Applied Ecology, 17, 2226–2228.
Witkowska, D., Ginter-Kramarczyk, D., Holderna-Odachowska, A., Budnik, I., Kaczorek, E., Lukaszewski, Z., & Zembrzuska, J. (2018). Biodegradation of oxyethylated fatty alcohols by bacterium Pseudomonas alcaligenes; AE biodegradation by Pseudomonas alcaligenes. Tenside, Surfactants, Detergents, 55, 43–48.
Zhou, J., Jiang, W., Ding, J., Zhang, X., & Gao, S. (2007). Effect of Tween 80 and beta-cyclodextrin on degradation of decabromodiphenyl ether (BDE-209) by White rot fungi. Chemosphere, 70, 172–177.
Funding
The project was supported by the Guangxi Science and Technology Program Project (2020AB22011), Sichuan Center for Rural Development Research (CR2001), the Open Fund of the Key Laboratory of Highway Engineering of Ministry of Education (Changsha University of Science & Technology) (KFJ180201), and the Open Research Fund of Guangxi Key Laboratory of Water Engineering Materials and Structures, Guangxi Institute of Water Resources Research (GXHRI-WEMS-2022-05).
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Shengwang Pan and Yuxiao Zhou. The first draft of the manuscript was written by Yuxiao Zhou, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Journal: Title in English 《Environmental Monitoring and Assessment》
Title of article: Assessment of the Efficiency of Immobilized Degrading Microorganisms in Removing the Organochlorine Pesticide Residues from Agricultural Soils.
Names of all contributing authors: Yuxiao Zhou, Shengwang Pan.
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Zhou, Y., Pan, S. Assessment of the efficiency of immobilized degrading microorganisms in removing the organochlorine pesticide residues from agricultural soils. Environ Monit Assess 195, 1274 (2023). https://doi.org/10.1007/s10661-023-11891-6
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DOI: https://doi.org/10.1007/s10661-023-11891-6