SpringerLink
Log in

Potato wilt caused by co-infection of Fusarium spp. and Verticillium dahliae in potato plants

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Potato Wilt is a common disease usually caused by either Verticillium dahliae or a Fusarium spp. In this study, we isolated and identified pathogens from diseased plants which were collected from different potato production areas along the foothills of Yinshan Mountain. The results showed 29 strains of Fusarium spp. and 37 strains of Verticillium dahliae isolated from 56 diseased potato plants. Among them, V. dahliae and Fusarium spp. were simultaneously isolated from 10 diseased plants. V. dahliae was the only fungal species co-isolated with Fusarium spp. The Fusarium spp. isolated were identified molecularly as F. oxysporum (5 strains), F. equiseti (2 strains), F. redolens (2 strains) and F. acuminatum (1 strain). Pathogenicity tests indicated that co-inoculation with both isolates of V. dahliae and Fusarium spp. on the same potato plants caused typical wilt symptoms, and both pathogens could be re-isolated from the diseased plants. This is the first report on co-infection of V. dahliae and Fusarium spp. causing potato wilt on single plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • An, X. M., Hu, J., Wu, J., Liu, W., & Meng, M. L. (2017). Overview of pathogen causing potato fusarium wilt. Chinese Potato, 05, 302–306.

    Google Scholar 

  • Azil, N., Stefańczyk, E., Sobkowiak, S., Chihat, S., Boureghda, H., & Liwka, J. (2021). Identification and pathogenicity of fusarium spp. associated with tuber dry rot and wilt of potato in Algeria. European Journal of Plant Pathology, 159, 495–509.

    Article  CAS  Google Scholar 

  • Bika, R., & Baysal-Gurel, F. (2021). Identification of fusarium commune, the causal agent of postharvest Zinnia meltdown disease in Tennessee. HortTechnology, 31(4), 432–438.

    Article  CAS  Google Scholar 

  • Cai, Y., Wang, C., Minghua, H., Cheng, J., Zhongyi, Q., & Chunmei, G. (2019). Identification of fusarium pathogens of Albizia julibrissin wilt and their cultural characteristics. Acta Agriculturae Zhejiangensis, 31(04), 588–599.

    Google Scholar 

  • Chen, A. C., Wei, Z.-Q., Ma, Y. Q., & Deng, C.-G. (2013). Isolation and identification of the pathogens causing potato verticillium wilt in Gansu. Journal of Plant Pathology, 43(4), 418–420.

    Google Scholar 

  • Chen, H., Xue, Y., Meng, M., Ma Zw, L. I. U. Z. H., & Hu, J. (2016). Pathogen identification and biological characteristics of potato wilt in Inner Mongolia. Chinese Potato Journal, 30(4), 226–234.

    Google Scholar 

  • Cui, L., Yang, C., Yang, L., **, M., & Wei, L. (2021). First report of fusarium equiseti causing fusarium wilt on potato (Solanum tuberosum) in China. Plant Disease, 105(7), 2013.

    Article  Google Scholar 

  • Daami-Remadi, M., & El Mahjoub, M. (2004). Emergence en Tunisie de Fusarium oxysporum f. sp. tuberosi agent deflétrissure vasculaire des plants et de pourriture sèche des tubercules de pomme de terre. EPPO Bulletin, 34, 407–411.

    Article  Google Scholar 

  • Dracatos, P. M., Haghdoust, R., Singh, D., & Park, R. F. (2018). Exploring and exploiting the boundaries of host specificity using the cereal rust and mildew models. The New Phytologist, 218, 453–462.

    Article  Google Scholar 

  • El-Shennawy, M. Z., Khalifa, E. Z., Ammar, M. M., Mousa, E. M., & Hafez, S. L. (2012). Biological control of the disease complex on potato caused by root-knot nematode and fusarium wilt fungus. Nematologia Mediterranea, 40, 169–172.

    Google Scholar 

  • FAOSTAT. (2021). http://www.fao.org/faostat/en/#data/QC. Accessed on 03.25.2021.

  • Gao, J., Yuanyuan, Z., Wang, K., Jian, Z., Gui, Z., & Jun, Z. (2016). Identification of sunflower wilt pathogen and its biological characteristics. Chinese Journal of Oil Crops, 38(2), 214.

    Google Scholar 

  • Isaac, I. (1953). A further comparative study of pathogenic isolates of Verticillium: V. nubilum Pethybr. And V. tricorpus sp.nov. Transactions of the British Mycological Society, 36(3), 180–195.

    Article  Google Scholar 

  • Jia, R., Xu, L., Yuanzheng, Z., Yaguang, H., Jun, Z., Guo, J., Wensheng, H., **e, R., Zhigang, H., & Yongxiu, Z. (2019). Identification of 37 potato varieties resistance to fusarium wilt caused by fusarium oxysporum. Chinese Potato, 33(05), 296–303.

    Google Scholar 

  • **g, R., Zhao, F. J., Liu, Y. F., Shang, W. J., Shen, R. Q., Guo, Q. Y., & Hu, S. P. (2019). Isolation and identification of potato verticillium wilt pathogen in Ningxia and its host range. Journal of Plant Pathology, 49(1), 11–19.

    Google Scholar 

  • Kang, L., Ruifang, J., Yuanyuan, Z., Gao, J., Yan, L., Jian, Z., Yan, Z., & Jun, Z. (2021). Identification of the resistance of different potato cultivars to potato verticillium wilt under indoor conditions. Journal of Inner Mongolia Agricultural University (Natural Science Edition), 42(01), 6–10.

    Google Scholar 

  • Krikun, J., & Orion, D. (1979). Verticillium wilt of potato: Importance and control. Phytoparasitica, 7(2), 107–116.

    Article  Google Scholar 

  • Li, S., Zhou, H., Lu, X., Nian, G., Guo, Q., Zhao, W., & Yan, L. (2018). Occurrence of potato Verticillium wilt and analysis of pathogenicity differentiation of the major pathogen in seven provinces of China. Acta Phytopathologica Sinica, 48(5), 656–665.

    Google Scholar 

  • Luo, A. H., Lu, L. Y., Hu, X. Y., **e, K. Z., & Liu, Y. Q. (2019). Studies on green and high-efficiency cultivation techniques of rain-fed staple food potato in mountainous region of cool and humidity. Chinese Potato, 33(4), 211–216.

    Google Scholar 

  • Maheshwari, T. U., Sharma, S. B., Reddy, D., & Haware, M. P. (1996). Co-infection of wilt-resistant chickpeas by fusarium oxysporum f. sp. ciceri and Meloidogyne javanica. Journal of Nematology, 27(4S), 649–653.

    Google Scholar 

  • Maqsood, A., Wu, H., Kamran, M., Altaf, H., Mustafa, A., Ahmar, S., & Chen, J. T. (2020). Variations in growth, physiology, and antioxidative defense responses of two tomato (Solanum lycopersicum L.) cultivars after co-infection of fusarium oxysporum and Meloidogyne incognita. Agronomy, 10(2), 159.

    Article  CAS  Google Scholar 

  • Nelson, P. E., Toussoun, T. A., & Cook, R. J. (Eds.). (1981). Fusarium: Diseases, biology and taxonomy. The Pennsylvania State University Press.

    Google Scholar 

  • O'Donnell, K., Rooney, A. P., Proctor, R. H., Brown, D. W., McCormick, S. P., Ward, T. J., Frandsen, R. J., Lysøe, E., Rehner, S. A., Aoki, T., Robert, V. A., Crous, P. W., Groenewald, J. Z., Kang, S., & Geiser, D. M. (2013). Phylogenetic analyses of RPB1 and RPB2 support a middle cretaceous origin for a clade comprising all agriculturally and medically important fusaria. Fungal Genetics and Biology, 52, 20–31.

    Article  CAS  Google Scholar 

  • Peng, X. W. (2003). The investigation of potato disease of Hebei province and research on major fungal diseases of potato. Hebei Agricultural University.

    Google Scholar 

  • Peng, S., Lu, X. L., Gao, F., Li, G. Y., & Li, H. (2008). Study on a new rapid inoculation method for Verticillium wilt and fusarium wilt of cotton. Cotton Sci, 20(3), 174–178.

    Google Scholar 

  • Pethybridge, G. H. (1919). Notes on some saprophytic species of fungi associated with diseased potato plants and tubers. Transactions of the British Mycological Society, 6(17), 104–120.

    Google Scholar 

  • Powelson, M. L., & Rowe, R. C. (1993). Biology and management of early dying of potatoes. Annual Review of Phytopathology, 31(1), 111–126.

    Article  CAS  Google Scholar 

  • Qian, H., Xu, P., Mengyu, C., & Light, G. (2017). Mixed infection by fusarium oxysporum and Alternaria tenuissima on sweet potato fusarium wilt. Journal of Plant Protection, 44(5), 867–868.

    Google Scholar 

  • Rafique, K., Kang, S., Aziz-ud-Din, Mahmood, T., Imran, Ullah, I., & Mahmood, H. (2020). First report of vascular wilt on lentil (Lens culinaris) caused by fusarium redolens in Pakistan. Plant Disease, 104(9), 2524–2524.

  • Sidhu, G., & Webster, J. W. (1977). Genetics of resistance in tomato to root-knot nematode-wilt fungus complex. Journal of Heredity, 65, 153–156.

    Article  Google Scholar 

  • Tekeoğlu, M., Özkilinç, H., Tunali, B., Küsmenoğlu, İ., & Weidong, C. H. E. N. (2017). Molecular identification of fusarium spp. causing wilt of chickpea and the first report of fusarium redolens in Turkey. Mediterranean Agricultural Sciences, 30(1), 27–33.

    Google Scholar 

  • Tian, S., Zhiming, Z., Jichen, L., Tan, Z., Wang, W., & Chaorui, D. (1995). Kinds and four new records of fungus disease of potato in Hebei province of China. Journal of Argicultural University of Hebei, 3, 59–62.

    Google Scholar 

  • Wagner, T., Gu, A., Duke, S. E., Bell, A. A., Magill, C., & Liu, J. (2020). Genetic diversity and pathogenicity of Verticillium dahliae isolates and their co-occurrence with Fusarium oxysporum f. sp. vasinfectum causing cotton wilt in **njiang, China. Plant Disease, 105(4), 978–985.

    Article  Google Scholar 

  • Wang, L., Riziwangguli, S., Li, K., Wang, H., & Luo, M. (2011). Kinds and five new records of fungus disease of potato in Urumqi and Changji area in **njiang. **njiang Agricultural Sciences, 48(2), 266–270.

    Google Scholar 

  • Wang, Y., Yang, C., **urong, C., Xue, L., & Jianhong, S. (2014a). Identification of potato wilt caused by fusarium mavenaceumand the biological characteristics. Plant Protection, 01, 48–53.

    Google Scholar 

  • Wang, L. L., Fu, G. H., Ma, J. G., Rziwangguli, L., & Li, K. M. (2014b). Isolation and identification of the pathogens causing Verticillium wilt of potato in Urumqi and Changji area, **njiang. **njiang Agricultural Sciences, 51, 667–672.

    CAS  Google Scholar 

  • Weiss, F. (1924). Survey for potato wilt in Pennsylvania and southern New York. American Journal of Potato Research, 1(11), 243–244.

    Article  Google Scholar 

  • Zhang, G. L., **e, Z. M., Feng, Z. L., Li, Q. S., Zhu, H. Q., Lv, N., & Sun, G. Q. (2020). Current occurrence of cotton wilt and yellow wilt disease in **njiang and its rapid isolation technology. Plant Protection, 46(03), 260–265.

    Google Scholar 

  • Zhu, Y. Y., Wang, Y. Y., & Bruce, R. L. (1999). PCR detection of Verticillium dahliae from diseased plants. Acta Phytopathologica Sinica, 29(3), 250–255.

    Google Scholar 

Download references

Acknowledgments

This research was funded by National Natural Science Foundation of China (31860495); Key Natural Science Foundation of Inner Mongolia Autonomous Region (2022ZD10); Key Technology Research Project in Inner Mongolia Autonomous Region (2019GG180) and Inner Mongolia Agriculture and Animal Husbandry Innovation Fund Project (2019CXJJNO7-2).

Author information

Author notes

  1. Ruifang Jia and Liru Kang contributed equally to this work.

Authors and Affiliations

  1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, China

    Ruifang Jia, Liru Kang, Mandela E. Addrah, Jian Zhang, Zhiwei Zhang, Jiecai Liu & Jun Zhao

  2. Plant Protection Department, Inner Mongolia Agriculture and Husbandry Academic Institution, Hohhot, China

    Limin Xu

  3. USDA Agricultural Research Service, Department of Plant Pathology, Washington State University, Pullman, WA, 99164, USA

    Weidong Chen

Authors
  1. Ruifang Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Liru Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mandela E. Addrah
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Limin Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhiwei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Weidong Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jiecai Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jun Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jiecai Liu or Jun Zhao.

Ethics declarations

Research involving human participants and/or animals

This research involved neither human participants nor animals.

Conflict of interest

The authors declare no conflict of interest.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jia, R., Kang, L., Addrah, M.E. et al. Potato wilt caused by co-infection of Fusarium spp. and Verticillium dahliae in potato plants. Eur J Plant Pathol 165, 305–315 (2023). https://doi.org/10.1007/s10658-022-02607-6

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-022-02607-6

Keywords

Search

Navigation