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Lasiodiplodia species diversity associated with coconut leaf blight and stem-end rot in Northeastern Brazil

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Abstract

Many abiotic and biotic factors can negatively affect the production and productivity of coconut crop** systems. The incidence of Lasiodiplodia species in crops can lead to significant economic losses due to the development of leaf blight and, consequently, both pre- and post-harvest stem-end rot of fruits. The management of these pathogens is complex, expensive and primarily depends, on the massive use of chemical products. In addition, decision making on effective control measures depends on the target pathogen. This work evaluated epidemiological aspects of Lasiodiplodia species, in association with the green dwarf coconut from Northeastern Brazil. The isolates were sampled in production areas located in the dry, sub-humid and humid climatic zones in the states of Pernambuco and Paraíba and were identified using multilocus phylogenetics with the tef1-α, ITS, tub2, and rpb2 regions. Lasiodiplodia theobromae was found in crops present in all three evaluated zones while L. pseudotheobromae was reported only in the dry zone of Pernambuco. All species were pathogenic to green dwarf coconut, with L. theobromae, L. pseudotheobromae and L. subglobosa being the most aggressive species. The recognition of L. theobromae, L. pseudotheobromae, L. brasiliensis and L. subglobosa, in coconut plants from Northeast Brazil, complements the scientific understanding about stem-end rot and leaf blight in coconut crop. Additionally, this study presents relevant information on the pathogenic characteristics of these species that can be used in future research into epidemiological factors, fungal resistance, phytosanitary management and genetic improvement.

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Data availability

The gene sequences, which support the results of species recognition, were deposited in GenBank and the other raw data, related to the results presented in this study, may be made available to the editorial board for conference, if requested.

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Acknowledgements

The authors thank the financial support offered by National Council of Technological and Scientific Development (CNPq). We also thank José Carlos Barbosa Nascimento; Itapirema Experimental Station of the Agronomic Institute of Pernambuco; we also thank to Phytobacteriology Laboratory of the Federal Rural University of Pernambuco – LAFBAC and to Laboratory of Bioinformatics and Evolutionary Biology, Federal University of Pernambuco – UFPE by support during field collections, availability of plant material used in the experiments, availability of physical structure and genetic analysis services, respectively.

Funding

No funding was received for conducting this study. All costs were subsidized by the authors themselves. During the research period, the main author received the aid of a doctoral scholarship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Universidade Federal Rural de Pernambuco (UFRPE), Dom Manoel de Medeiros Street, Dois Irmãos, Recife, PE, CEP: 52171-900, Brazil

    Iwanne Lima Coelho, Willie Anderson dos Santos Vieira, Marcos Paz Saraiva Câmara & Delson Laranjeira

  2. Instituto Agronômico de Pernambuco (IPA), General San Martin Avenue, 1371, Bongi, Recife, PE, CEP: 50761-00, Brazil

    Tereza Cristina de Assis & Luiz Gonzaga Biones Ferraz

  3. Universidade Federal de Pernambuco (UFPE), Prof. Moraes Rego Avenue, 1235, Cidade Universitária, Recife, PE, CEP: 50670-901, Brazil

    Moisés Thiago de Souza Freitas, Valdir de Queiroz Balbino & Rejane Pereira Neves

  4. Louisiana State University AgCenter, Baton Rouge, LA, USA

    Vinson P. Doyle

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  1. Iwanne Lima Coelho
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  2. Tereza Cristina de Assis
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Coelho, I.L., de Assis, T.C., Ferraz, L.G.B. et al. Lasiodiplodia species diversity associated with coconut leaf blight and stem-end rot in Northeastern Brazil. Eur J Plant Pathol 162, 45–61 (2022). https://doi.org/10.1007/s10658-021-02383-9

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