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Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques

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Abstract

The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.

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Acknowledgments

This work was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia (Grants No. 173026 and 31018)

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Authors and Affiliations

  1. Institute for Plant Protection and the Environment, Belgrade, 11000, Serbia

    Žarko Ivanović, Tatjana Popović & Veljko Gavrilović

  2. Department of Laboratory Methods and Diagnostics, Dutch General Inspection Service (NAK), Emmeloord, 8300, BC, The Netherlands

    Jaap Janse

  3. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, 11000, Serbia

    Milan Kojić

  4. Faculty of Biology, University of Belgrade, Belgrade, 11000, Serbia

    Slaviša Stanković & Djordje Fira

Authors
  1. Žarko Ivanović
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  2. Tatjana Popović
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  3. Jaap Janse
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  4. Milan Kojić
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  5. Slaviša Stanković
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  6. Veljko Gavrilović
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  7. Djordje Fira
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Correspondence to Djordje Fira.

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Ivanović, Ž., Popović, T., Janse, J. et al. Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. Eur J Plant Pathol 141, 133–145 (2015). https://doi.org/10.1007/s10658-014-0531-5

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