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Isolation and expression analysis of a CBF transcriptional factor gene from the mangrove Bruguiera gymnorrhiza

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Abstract

The present work isolated a CBF/DREB1 gene from mangrove Bruguiera gymnorrhiza (BgCBF1) and compared its expression levels in various tissues under normal condition and cold stress, and in leaves exposed to various environmental stimuli. Results showed that the BgCBF1 deduced protein showed almost 100% similarities to that of AcCBF1 from Aegiceras corniculatum and AmCBF1 from Avicennia marina. Real-time quantitative PCR analysis showed that BgCBF1 gene displayed constitute expression in leaf, stem and root samples of plantlets under normal condition, but with different expression levels and tissue preference. When exposed to cold, BgCBF1 could be rapidly, slightly and transiently induced in all tissues. Furthermore, the BgCBF1 gene in leaves displayed a transient and small induction after salt and drought (PEG) exposure, while exhibited relatively high up-regulated expression after the phytohormone abscisic acid (ABA) treatment. These results suggest that the BgCBF1 gene may participate in the ABA mediated development and protection of plant against cold and drought. Further studies on its promoters and downstream genes will be needed to better understand its functions.

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Acknowledgements

This research was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDA23050200), the National Natural Science Foundation of China (No.U1901211, No.41430966 and No.41876126), the National Key Research and Development Plan (No. 2017FY100700) and the International Partnership Program of Chinese Academy of Sciences (No. 133244KYSB20180012).

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Authors and Affiliations

  1. State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Ya-Lan Peng, You-Shao Wang, Jiao Fei, Hao Cheng & Cui-Ci Sun

  2. Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen, 518121, China

    Ya-Lan Peng, You-Shao Wang, Jiao Fei, Hao Cheng & Cui-Ci Sun

  3. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, 510301, China

    You-Shao Wang & Cui-Ci Sun

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  1. Ya-Lan Peng
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Correspondence to You-Shao Wang.

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Peng, YL., Wang, YS., Fei, J. et al. Isolation and expression analysis of a CBF transcriptional factor gene from the mangrove Bruguiera gymnorrhiza. Ecotoxicology 29, 726–735 (2020). https://doi.org/10.1007/s10646-020-02215-2

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