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Exogenous sucrose influences antioxidant enzyme activities and reduces lipid peroxidation in water-stressed cucumber leaves

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Biologia Plantarum

Abstract

To investigate whether exogenous sucrose can protect cucumber from water stress, cucumber (Cucumis sativus L.) seedlings were pretreated with 90 mM sucrose or 90 mM mannitol for 1 d and then were dehydrated with 10 % (m/v) polyethylene glycol (PEG) 6000 for further 1 d. Dehydration inhibited plant growth and decreased osmotic potential and relative water content (RWC) in leaves. The pretreatment with 90 mM sucrose further reduced the osmotic potential but increased the RWC and alleviated the growth inhibition. Compared with the PEG treatment alone, the combination of sucrose + PEG increased the activities of superoxide dismutase, guaiacol peroxidase, glutathione reductase, dehydroascorbate reductase, monodehydroascorbate reductase, ascorbate peroxidase, and glutathione peroxidase, and elevated the content of endogenous sucrose, glucose, and fructose together with the activities of soluble acid invertase and neutral invertase. This was in accordance with the enhanced transcription of genes encoding copper/zinc superoxide dismutase, guaiacol peroxidase, and glutathione reductase. Furthermore, the sucrose pretreatment decreased the content of malondialdehyde and hydrogen peroxide and increased the content of ascorbate, reduced glutathione, and proline under the dehydration. Taken together, the pretreatment with 90 mM sucrose, but much less with mannitol, induced antioxidants, proline, and soluble sugars and thus reduced dehydration-caused damage to the cucumber seedlings.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbate

DHAR:

dehydroascorbate reductase

GPX:

guaiacol peroxidase

GR:

glutathione reductase

GSH:

glutathione

GSH-Px:

glutathione peroxidase

MDHAR:

monodehydroascorbate reductase

NI:

neutral invertase

PEG:

polyethylene glycol

ROS:

reactive oxygen species

RWC:

relative water content

SAI:

soluble acid invertase

SOD:

superoxide dismutase

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Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiao Tong University/State Key Laboratory of Microbial Metabolism, Shanghai, 200240, P. R. China

    Y. -Y. Cao

  2. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China

    Y. -Y. Cao, M. -T. Yang, S. -Y. Chen, Z. -Q. Zhou, X. Li, X. -J. Wang & J. -G. Bai

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  1. Y. -Y. Cao
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Correspondence to J. -G. Bai.

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The first two authors contributed equally to this paper.

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Cao, Y.Y., Yang, M.T., Chen, S.Y. et al. Exogenous sucrose influences antioxidant enzyme activities and reduces lipid peroxidation in water-stressed cucumber leaves. Biol Plant 59, 147–153 (2015). https://doi.org/10.1007/s10535-014-0469-7

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  • DOI: https://doi.org/10.1007/s10535-014-0469-7

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