Abstract
Endophytic fungi associated with plants may contain undiscovered bioactive compounds. Under standard laboratory conditions, most undiscovered compounds are inactive, whereas their production could be stimulated under different cultivation conditions. In this study, six endophytic fungi were isolated from the bark of Koelreuteria paniculata in Quancheng Park, **an City, Shandong Province, one of which was identified as a new subspecies of Aureobasidium pullulans, named A. pullulans KB3. Additionally, metabolomic tools were used to screen suitable media for A. pullulans KB3 fermentation, and the results showed that peptone dextrose medium (PDM) was more beneficial to culture A. pullulans KB3 for isolation of novel compounds. Sphaerolone, a polyketone compound, was initially isolated from A. pullulans KB3 via scaled up fermentation utilizing PDM. Additionally, the whole-genome DNA of A. pullulans KB3 was sequenced to facilitate compound isolation and identify the biosynthesis gene clusters (BGCs). This study reports the multi-omics (metabolome and genome) analysis of A. pullulans KB3, laying the foundation for discovering novel compounds of silent BGCs and identifying their biosynthesis pathway.
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Data Availability
The data presented in this study are available on request from the corresponding author.
Code Availability
Endophytic fungal ITS sequences of the rDNA gene region obtained in this study were deposited in GenBank (KB1: OQ996271; KB2: OQ996272; KB3: OQ996273; KB4: OQ996274; KB5: OQ996275; KB6: OQ996276).
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Funding
This study was supported by grants from Basal Research Fund of Hebei Academy of Sciences (2023PF02), Science and technology planning project of Hebei Academy of Sciences (23312), High-level talents training and funding projects of Hebei academy of sciences (2022G01), and Natural Science Foundation of Shandong Province (ZR2019PC030).
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Conceptualization was performed by Hong-Wei Liu. Formal analysis, investigation, material preparation, data collection, and analysis were performed by Ying-Jie Wang, Qing-**a Yang, Ya-Na Wang, Yang Zhang, Jia-Nuo He, Wen-Ya Zhao, and Li-** Zhang. Funding acquisition was supported by Hong-Wei Liu. Methodology was performed by Shuai Wang. The first draft of the manuscript was written by Shuai Wang and Jia-Nuo He, the final draft of the manuscript was confirmed by Hong-Wei Liu and Shuai Wang. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Wang, S., He, JN., Wang, YJ. et al. Metabolome and Genome Analysis of a Novel Endophytic Fungus Aureobasidium pullulans KB3: Discovery of Polyketones and Polyketone Biosynthesis Pathway. Biochem Genet (2024). https://doi.org/10.1007/s10528-024-10866-7
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DOI: https://doi.org/10.1007/s10528-024-10866-7