Abstract
Stroke is a cerebrovascular disease with impaired nerve function. Long non-coding RNA (lncRNA) is considered to be an important regulator of various diseases. Nevertheless, the role of lncRNA small nucleolar RNA host gene 15 (SNHG15) in cerebral ischemia injury induced by stroke is still unclear. Cell-counting kit 8 assay and flow cytometry were used to detect cell viability and apoptosis, respectively. The caspase3 activity of cells was measured using Caspase3 Activity Assay Kit. Besides, the protein levels of apoptosis markers and TCCD-induced poly (ADP)-ribose polymerase (TIPARP) were determined using western blot analysis. Moreover, quantitative real-time polymerase chain reaction was employed to examine the relative expression of SNHG15 and miR-9-5p. Furthermore, dual-luciferase reporter assay was used to assess the interaction between miR-9-5p and SNHG15 or TIPARP. In addition, biotin-labeled RNA pull-down assay was performed to evaluate the interaction between miR-9-5p and SNHG15 further. Middle cerebral artery occlusion (MCAO) model was constructed to further explore the role of SNHG15 in neuronal injury in vivo. Our data showed that oxygen and glucose deprivation (OGD) could induce N-2a cell injury and enhance SNHG15 expression. Silenced SNHG15 could promote the viability and suppress the apoptosis of OGD-induced N-2a cells. Also, SNHG15 knockdown also could alleviate the neuronal injury of MCAO mice. Mechanistically, SNHG15 could sponge miR-9-5p, and miR-9-5p could target TIPARP. Further experiments revealed that miR-9-5p inhibition or TIPARP overexpression could reverse the suppressive effect of SNHG15 knockdown on OGD-induced N-2a cell injury. Our findings indicated that SNHG15 knockdown inhibited neuronal injury through the miR-9-5p/TIPARP axis, suggesting that SNHG15 might be a potential target for cerebral ischemia injury induced by stroke.
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Abbreviations
- SNHG15:
-
Small Nucleolar RNA Host Gene 15
- TIPARP:
-
TCCD-induced Poly (ADP)-Ribose Polymerase
- OGD:
-
Oxygen and Glucose Deprivation
- MCAO:
-
Middle Cerebral Artery Occlusion
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This work was funded by Yancheng Development Project of Medical Science and Technology in 2020 (YK2020061).
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10528_2021_10121_MOESM1_ESM.tif
Supplementary Fig. 1 Selection of candidate miRNA for SNHG15 and candidate targets for miR-9-5p. (A) Biotin-labeled RNA pull-down assay was used to analyze the enrichment of each candidate miRNA in SNHG15 probe and Oligo probe. (B) The mRNA expression was measured by qRT-PCR in N-2a cells transfected with miR-9-5p mimic or miR-NC. ***P < 0.001. Supplementary file1 (TIF 210 KB)
10528_2021_10121_MOESM2_ESM.tif
Supplementary Fig. 2 Effect of SNHG15 knockdown on neuronal injury. (A) The expression of SNHG15 in the brain tissues of each group was measured by qRT-PCR. (B) Infarct volume of each group was assessed. (C) Longa scoring system was used to analyze the neurological scores. (D) The expression of miR-9-5p in the brain tissues of each group was determined by qRT-PCR. (E) TIPARP protein expression in the brain tissues of each group was detected by WB analysis. **P < 0.01. Supplementary file2 (TIF 409 KB)
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Shen, B., Wang, L., Xu, Y. et al. Knockdown of lncRNA SNHG15 Ameliorates Oxygen and Glucose Deprivation (OGD)-Induced Neuronal Injury via Regulating the miR-9-5p/TIPARP Axis. Biochem Genet 60, 755–769 (2022). https://doi.org/10.1007/s10528-021-10121-3
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DOI: https://doi.org/10.1007/s10528-021-10121-3