We studied the dependence of the levels of Pro-Gly-Pro-Leu peptide and its metabolites (Gly-Pro-Leu, Pro-Gly-Pro, Gly-Pro and Pro-Gly) from the time of administration in the hippocampus, cerebellum, and cerebral cortex of rats. After intravenous injection of Pro-Gly-Pro-Leu, the maximum concentration of metabolites in the rat brain was found in 20-min sample (0.026% from the amount of introduced labeled tetrapeptide); it was by 2.6 times higher that after intranasal administration. The calculated ratios of the peptide content (AUC) in brain structures for Pro-Gly-Pro-Leu and its metabolites after intranasal and intravenous injection were in most cases >1; hence, the levels of these peptides in the cerebellum, hippocampus, and brain cortex after intravenous injection were slightly higher than after intranasal administration. From these data, content of Pro-Gly-Pro-Leu and its metabolites per 1 g of the cerebellum, hippocampus and cortex was calculated: the maximum concentrations of Pro-Gly-Pro-Leu in the cerebellum, hippocampus, and cortex were 15.63, 18.48, and 2.95 pmol/g, respectively.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 158, No. 7, pp. 43-48, July, 2014
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Shevchenko, K.V., V’yunova, T.V., Andreeva, L.A. et al. Proteolysis of Pro-Gly-Pro-Leu in the Hippocampus, Cerebellum, and Cerebral Cortex in Rats after Intravenous Injection. Bull Exp Biol Med 158, 37–41 (2014). https://doi.org/10.1007/s10517-014-2686-7
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DOI: https://doi.org/10.1007/s10517-014-2686-7