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Effects of exogenous non-starch polysaccharide-degrading enzymes in diets containing Gracilaria lemaneiformis on white-spotted snapper Lutjanus stellatus Akazaki

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Abstract

A feeding experiment was conducted to investigate the effects of exogenous non-starch polysaccharide (NSP)-degrading enzymes in diets containing Gracilaria lemaneiformis (GL) on growth performance and digestive enzyme activities of white-spotted snapper Lutjanus stellatus Akazaki (initial mass 8.0 ± 0.1 g). A basal diet (D0) containing a mixed protein source (fish meal, soybean meal and GL meal) was used as the control. Two diets supplemented with 0.5 g (D1) and 1 g (D2) exogenous NSP-degrading enzymes per kilogram of diet were formulated. Each diet was assigned to triplicate groups of fish in a total of nine floating sea cages (270 fish, 30 fish per cage). After a 60-day feeding trail, significantly higher weight gain, specific growth ratio and feed efficiency ratio were observed in fish fed D2 diet compared to those of control (P < 0.05). Body lipid, moisture and ash contents were not significantly affected by NSP-degrading enzyme supplementation, but significantly higher protein content was noticed in fish fed D2 diet compared to that of control (P < 0.05). The amylase activity in the stomach and intestine was significantly higher in fish fed D2 diet (P < 0.05), but no significant differences were observed in pepsin and lipase activities in the stomach or trypsin and lipase activities in the intestine between all treatments. The results suggested that addition of 1 g kg−1 NSP-degrading enzymes in diet could efficiently improve seaweed feed utilization and growth performance of white-spotted snapper fish.

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Acknowledgments

This study was supported by Grant Nos. 41276179 and 41276179 from National Natural Science Foundation of China, Grant No. S2011030005257 from Guangdong Natural Science Foundation and Grant No. 2010B020201015 from Guangdong Science and Technology Funding Scheme, and the authors thank Prof. Chiju Wei, of the College of Science, Shantou University, China, for reviewing the manuscript and his helpful suggestions.

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Authors and Affiliations

  1. College of Animal Science, Yangtze University, **gzhou, 434023, People’s Republic of China

    Dashi Zhu & **aobo Wen

  2. College of Animal Science, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Dashi Zhu

  3. Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, People’s Republic of China

    Shengkang Li, **ongzhi Xuan & Yuanyou Li

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  1. Dashi Zhu
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  2. **aobo Wen
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Correspondence to **aobo Wen.

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Zhu, D., Wen, X., Li, S. et al. Effects of exogenous non-starch polysaccharide-degrading enzymes in diets containing Gracilaria lemaneiformis on white-spotted snapper Lutjanus stellatus Akazaki. Aquacult Int 24, 491–502 (2016). https://doi.org/10.1007/s10499-015-9940-5

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