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Fluid pum** and cells separation by DC-biased traveling wave electroosmosis and dielectrophoresis

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Abstract

Fluid pum** in microchips using electrokinetic methods has been a hot area of research. This paper mainly investigates effects of DC offset imposed on traveling wave (TW) signal on electroosmotic flow above a spiral electrode array with 800 µm wavelength. The traveling wave voltage with different DC offsets was applied, and four cases were analyzed by superimposing consecutive images. Experiment results indicate that symmetric electrode array energized with DC-biased TW signal can not only generate a prominent improvement in flow rates, but also be capable of altering the flow direction by changing the polarity of electrical signal. Furthermore, such a device can also be used as an effective means to manipulate and separate PS microbeads and cells on their own for very small and non-flowing sample volumes in terms of the combination of the conventional dielectrophoresis (cDEP) forces and traveling wave DEP (twDEP) forces by properly choosing the parameters associated with the Clausius–Mossotti factor (K(w)). Through adjusting the applied frequencies, we successfully separated yeast cells from a mix containing PS microspheres based on the combination of cDEP and twDEP, providing new opportunities for integration with a charge-coupled device for various biomedical diagnostic devices.

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Acknowledgements

This work is supported by the National Natural Science Foundation of China (Nos. 51305106, 11672095 and 11372093), the Fundamental Research Funds for the Central Universities (Nos. HIT. NSRIF. 2014058 and HIT. IBRSEM. 201319), Self-Planned Task (Nos. 201510B and SKLRS201606C) of State Key Laboratory of Robotics and System (HIT) and the Programme of Introducing Talents of Discipline to Universities (No. B07018).

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Authors and Affiliations

  1. School of Mechatronics Engineering, Harbin Institute of Technology, West Da-zhi Street 92, Harbin, 150001, Heilongjiang, People’s Republic of China

    Yupan Wu, Yukun Ren, Ye Tao & Hongyuan Jiang

  2. State Key Laboratory of Robotics and System, Harbin Institute of Technology, West Da-zhi Street 92, Harbin, 150001, Heilongjiang, People’s Republic of China

    Yukun Ren & Hongyuan Jiang

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  1. Yupan Wu
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  2. Yukun Ren
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  3. Ye Tao
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  4. Hongyuan Jiang
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Correspondence to Yukun Ren or Hongyuan Jiang.

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This article is part of the topical collection “2016 International Conference of Microfluidics, Nanofluidics and Lab-on-a-Chip, Dalian, China” guest edited by Chun Yang, Carolyn Ren and **angchun Xuan.

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Wu, Y., Ren, Y., Tao, Y. et al. Fluid pum** and cells separation by DC-biased traveling wave electroosmosis and dielectrophoresis. Microfluid Nanofluid 21, 38 (2017). https://doi.org/10.1007/s10404-017-1862-2

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