Abstract
From 2007 to 2013, a disease of Welsh onion, causing leaf sheath rot and concomitant death of outer leaves was found in 20 fields in Hokkaido, Japan. We obtained 20 Rhizoctonia isolates from diseased tissues and identified them based on the number of nuclei, hyphal fusion reactions, and molecular techniques using specific PCR primers and sequence of the rDNA-ITS region. The 20 isolates consisted of 16 multinucleate and four binucleate isolates. Of the multinucleate isolates, five were found to be so far unknown and designated here as Rhizoctonia solani AG-4 hybrid subgroup between HG-I and HG-II. Others were identified as AG-1 IB (three isolates), AG-2-2 IIIB (two isolates), AG-4 HG-I (two isolates), AG-1 IC (one isolate), AG-2-1 (one isolate), AG-4 HG-II (one isolate) and AG-5 (one isolate). All four binucleate isolates were binucleate Rhizoctonia AG-U. Original symptoms were reproduced on all plants inoculated with these isolates. Thus, we revealed that as many as nine taxa of Rhizoctonia spp. were associated with the disease. This is the first report of leaf sheath rot of Welsh onion caused by Rhizoctonia spp.
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Acknowledgements
This study was supported in part by Grants-in-Aid for Regional R&D Proposal-Based Program from Northern Advancement Center for Science & Technology of Hokkaido Japan. For help with this work, we thank staff members of Hokkaido Prefectural Oshima, Sorachi and Iburi Agricultural Extension Centers, Japan. We thank A. Sumino (Hokkaido Research Organization) for valuable comments and Dr. N. Matsumoto (Hokkaido University, Japan) for critically reading the manuscript and correcting the English.
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The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases as accessions LC090068–090073.
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Misawa, T., Kurose, D. & Kuninaga, S. First report of leaf sheath rot of Welsh onion caused by nine taxa of Rhizoctonia spp. and characteristics of the pathogens. J Gen Plant Pathol 83, 121–130 (2017). https://doi.org/10.1007/s10327-017-0706-y
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DOI: https://doi.org/10.1007/s10327-017-0706-y