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Photodynamic therapy mediated by nanoparticles Aluminum Chloro Phthalocyanine in oral squamous carcinoma cells

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Abstract

The aim of this study is to investigate the antineoplastic potential of photodynamic therapy (PDT) mediated by an aluminum-phthalocyanine chloride nanoemulsion (AlPc-NE), against an oral squamous cell carcinoma (OSCC) cell line in vitro. Both OSCC (SCC9) and A431 cell lines were studied in vitro. Four study groups were used: Group 1 (phosphate-buffered saline [PBS]), Group 2 (PBS + 28.3 J/cm2 irradiation), Group 3 (AlPc-NE alone), and Group 4 (AlPc-NE + 28.3 J/cm2 irradiation). To test the effect of PDT with AlPc-NE, cell viability, migration, and cell death assays were performed. Moreover, the expressions of Ki-67 and TP53 were evaluated using immunoassays. The results showed that PDT mediated by all AlPc-NE concentrations evaluated (i.e., 0.7, 0.35, and 0.17 nM AlPc) significantly reduced the viability of SCC9 cells. Migration and cell death assays also revealed that PDT with AlPc-NE significantly reduced the rate of migration and increased cell death compared to the control groups. In addition, it was found that PDT with AlPc-NE reduced Ki-67 and mutated TP53 immunoexpression. PDT with AlPc-NE is effective in reducing the viability and migration of SCC9. Moreover, PDT with AlPc-NE nanoemulsions reduces the cell proliferation and expression of mutant TP53.

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Abbreviations

PDT:

Photodynamic therapy

AlPc-NE:

Aluminum-phthalocyanine chloride nanoemulsion

OSCC:

Oral squamous cell carcinoma

SCC9:

OSCC cell line

PS:

Photosensitizer drug

ROS:

Reactive oxygen species

PVM/MA:

Poly(vinyl methyl ether/maleic anhydride) (polymer nanoparticle)

LED:

Light-emitting diode

INCT:

National Institutes of Science and Technology

CNPq:

National Council for Scientific and Technological Development

DMEM/Ham’s F-12:

Dulbecco’s modified Eagle’s medium/nutrient mixture F-12

MTT:

3-(4,5-Dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide

AO/EB:

Acridine orange/ethidium bromide

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Funding

This study was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and the Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG). Dr. Guimarães, Dr. Dr. Santos, and Dr. de Paula are research fellows of the CNPq.

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Authors and Affiliations

  1. Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil

    Lilian Mendes Borburema Cangussu, Ludmilla Regina de Souza, Marcela Gonçalves de Souza, Lucyana Conceição Farias, Alfredo Maurício Batista de Paula & André Luiz Sena Guimarães

  2. Department of Physical Education, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil

    Renato Sobral Monteiro Junior

  3. Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Federal District, Brazil

    Luis Alexandre Muehlmann

  4. Institute of Physics of the University of Brasilia, University of Brasilia, Federal District, Brazil

    Paulo Narcizo de Souza

  5. Institute of Agricultural Sciences, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil

    Sérgio Henrique Sousa Santos

  6. Dilson Godinho Hospital, Montes Claros, Minas Gerais, Brazil

    André Luiz Sena Guimarães

Authors
  1. Lilian Mendes Borburema Cangussu
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  2. Ludmilla Regina de Souza
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  7. Lucyana Conceição Farias
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  8. Sérgio Henrique Sousa Santos
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  10. André Luiz Sena Guimarães
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Corresponding author

Correspondence to André Luiz Sena Guimarães.

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Ethical approval

All performed procedures involving human participants were conducted following the ethical standards of the institutional and national research committees, the 1964 Helsinki declaration, and its later amendments, or comparable ethical standards. Ethical approval for this study (No. 52760016.9.0000.5146) was obtained from the Institutional Review Board.

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Cangussu, L.M.B., de Souza, L.R., de Souza, M.G. et al. Photodynamic therapy mediated by nanoparticles Aluminum Chloro Phthalocyanine in oral squamous carcinoma cells. Lasers Med Sci 37, 2509–2516 (2022). https://doi.org/10.1007/s10103-022-03517-z

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