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High levels of Staphylococcus aureus and MRSA carriage in healthy population of Algiers revealed by additional enrichment and multisite screening

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Abstract

The purpose of the research is to characterize Staphylococcus aureus colonization in healthy population of Algiers, to assess the impact on diagnostic performance of systematic additional broth enrichment, and to ascertain the additional benefits of multiple site screening. In order to more accurately determine the prevalence of S. aureus colonization, the swab specimens from multiple screening sites were incubated in brain-heart broth before agar plating. From 2009 to 2011, 1176 samples were collected from 459 participants (201 adults and 258 children). The additional enrichment detection step significantly increased S. aureus detection rates (p < 0.0001). S. aureus nasal detection was positive in 37.8% of adults, and the addition of throat samplings significantly increased the S. aureus detection rate up to 54.7% (p < 0.001). S. aureus nasal detection was positive in 37.6% of children. The addition of throat samplings in children significantly increased the S. aureus detection rate up to 53.1% (p < 0.001) and that of anal samplings up to 59.7%. The overall prevalence of methicillin-resistant S. aureus was 5.2% (3% of adults and 7% of children). spa ty** of all isolates revealed a diverse but strongly clonal S. aureus population structure. This approach involving multiple anatomical sampling sites and an additional enrichment of the swabs before conventional culture significantly increases the detection rate of S. aureus carriers and may prove valuable to improve global S. aureus infection prevention.

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Acknowledgments

We are grateful to all the staff of the Microbiology Department of the CHU Mustapha Bacha Algiers, especially Mr. Tazir Mohamed, Bachtarzi Mohamed-Azzedine, Benbouzid Djazia, Belghit Yousra Bouchra, and Nasseh Chahinez, the USTHB students, as well as all the staff of the French National Reference Center for Staphylococci in Lyon.

Funding

This study was supported by Santé Publique France (Institut national de la santé et de la recherche médicale).

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Authors and Affiliations

  1. Service de Microbiologie, Centre Hospitalo-Universitaire Mustapha Bacha, Algiers, Algeria

    Kenza Antri & Nadjia Ramdani-Bouguessa

  2. Département de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari-Boumediène, Algiers, Algeria

    Kenza Antri

  3. Institut des Sciences Vétérinaires, Université Blida1, 09000, Blida, Algeria

    Madjid Akkou

  4. CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France

    Coralie Bouchiat, Michèle Bes, Patricia Martins-Simoes, Olivier Dauwalder, Anne Tristan, Hélène Meugnier, Jean-Philippe Rasigade, Jerome Etienne, François Vandenesch & Frédéric Laurent

  5. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France

    Coralie Bouchiat, Michèle Bes, Patricia Martins-Simoes, Olivier Dauwalder, Anne Tristan, Hélène Meugnier, Jean-Philippe Rasigade, Jerome Etienne, François Vandenesch & Frédéric Laurent

  6. Laboratoire d’analyses Médicales, Baraki, Algiers, Algeria

    Nadjia Ramdani-Bouguessa

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  1. Kenza Antri
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  2. Madjid Akkou
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Correspondence to Madjid Akkou.

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The authors declare that they have no conflict of interest.

Ethical approval

The study protocol was approved by the local ethics committee (Ethics Committee of Mustapha Bacha Hospital).

Informed consent

A written consent was obtained from the participants or their legal representatives (for participants younger than 16 years) before collecting samples.

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Antri, K., Akkou, M., Bouchiat, C. et al. High levels of Staphylococcus aureus and MRSA carriage in healthy population of Algiers revealed by additional enrichment and multisite screening. Eur J Clin Microbiol Infect Dis 37, 1521–1529 (2018). https://doi.org/10.1007/s10096-018-3279-6

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