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Effects of modified-BHI medium on the growth and metabolites of Akkermansia muciniphila

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Abstract

Akkermansia muciniphila (Akk) has recently become popular due to its therapeutic effect on various diseases. However, Akk’s high-density cultivation is difficult due to its anaerobic characteristics. Therefore, Akk was cultured with modified brain–heart infusion (M-BHI) to reach 1011 CFU/mL. 1H-NMR determined the metabolites of Akk and validated them by an amino acid analyzer. Compared to the BHI, Akk significantly up-regulated lactate, histidine, fumaric acid, cytidine, threonine, arginine, and hydroxyproline in the M-BHI and significantly down-regulated methionine, trimethylamine, and sarcosine. Regarding pathway enrichment analysis, histidine metabolism, arginine and proline metabolism, cysteine and methionine metabolism mainly regulate differential metabolites. In addition, M-BHI alters the metabolic profile by affecting Akk’s involvement in amino acid metabolism remodeling. Changed metabolites showed that Akk fermentation in M-BHI may play a physiological role in regulating immune homeostasis and reducing risk factors related to diseases. Therefore, M-BHI provides a promising reference for Akk cultivation in future industrial preparation.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31871802) and the Guangxi Key Research and Development Program (AB18221065).

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Authors and Affiliations

  1. College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China

    Qinren Zhang, Yupan Zhou, Fan Zhou, Pengcheng Chi & Quanyang Li

  2. Guangxi University Hospital, Guangxi University, Nanning, 530004, China

    Qianzu He & Haiyan Zhao

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Zhang, Q., Zhou, Y., He, Q. et al. Effects of modified-BHI medium on the growth and metabolites of Akkermansia muciniphila. Food Sci Biotechnol 33, 1921–1930 (2024). https://doi.org/10.1007/s10068-023-01492-x

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