Abstract
MV was reported to have beneficial effects in ameliorating insulin resistance in db/db mice, but the intrinsic mechanisms for glucose homeostasis are unclear. This study examined the anti-diabetic mechanism of MV using HepG2 cells and C57BL/KsJ-db/db mice. MV increased insulin sensitivity by promoting insulin-dependent glucose uptake and activating glycogen accumulation in HepG2 cells. Furthermore, the glucose homeostasis was enhanced in db/db mice administered 1 mg/kg/day of MV for eight weeks by activating the IRS-1/PI3K/Akt and AMPK pathways in the skeletal muscle and liver tissue. In addition, MV promoted glycogen synthesis by regulating the key enzymes, including GSK-3β and GS, and suppressed gluconeogenesis by inhibiting the mRNA expressions of G6pase and PEPCK. These findings show that MV regulates both signaling pathways and improves the glucose metabolism disorder. Thus, MV might be an alternative functional food or nutraceutical in ameliorating T2DM.
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Abbreviations
- MV:
-
Monascus-fermented grain vinegar
- IRS-1:
-
Insulin receptor substrate-1
- PI3K:
-
Phosphatidylinositol 3-kinase
- Akt:
-
Protein kinase B
- AMPK:
-
AMP-activated protein kinase
- GLUT4:
-
Glucose transporter type 4
- GSK-3β:
-
Glycogen synthase kinase-3β
- GS:
-
Glycogen synthase
- G6pase:
-
Glucose-6-phosphatase
- PEPCK:
-
Phosphoenolpyruvate carboxykinase
- GAPDH:
-
Glyceraldehyde 3-phosphate dehydrogenase
- T2DM:
-
Type 2 diabetes mellitus
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Acknowledgements
This study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1F1A1054590).
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Lee, YW., Pyo, YH. Monascus-fermented grain vinegar enhances glucose homeostasis through the IRS-1/PI3K/Akt and AMPK signaling pathways in HepG2 cell and db/db mice. Food Sci Biotechnol 31, 1583–1591 (2022). https://doi.org/10.1007/s10068-022-01146-4
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DOI: https://doi.org/10.1007/s10068-022-01146-4