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Light-hormone interaction in the red-light-induced suppression of photomorphogenesis in rice seedlings

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Abstract

Red light perceived by the shoot bottom suppresses photomorphogenesis in rice seedlings mediated by phytochrome A. Shoots of these seedlings grown in red light having their shoot bottom exposed were deficient in chlorophyll and accumulated high concentration of trans-zeatin riboside. However, reduced presence of isopentynyl adenosine, dihydrozeatin riboside was observed in shoots of red-light-grown non-green seedlings in comparison to green seedling. The message abundance of cytokinin receptor (OsHK5), transporters (OsENT1, OsENT2), and response regulators (OsRR4, OsRR10) was downregulated in these red-light-grown non-green seedlings. Attenuation of greening process was reversed by application of exogenous cytokinin analogue, benzyladenine, or supplementing red light with blue light. In the same vein, the suppression of gene expression of cytokinin receptor, transporters, and type-A response regulators was reversed in red-light-grown seedlings treated with benzyladenine suggesting that the disarrayed cytokinin (CK) signaling cascade is responsible for non-greening of seedlings grown in red light. The reversal of red-light-induced suppression of photomorphogenesis by blue light and benzyladenine demonstrates the interaction of light and cytokinin signaling cascades in the regulation of photomorphogenesis. Partial reversal of greening process by exogenous application of benzyladenine suggests, apart from CKs perception, transportation and responsiveness, other factors are also involved in modulation of suppression of photomorphogenesis by red light.

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Acknowledgments

This work was supported by grants from the University Grants Commission, India [No. F. 31-168/2005(SR)], to BCT and PURSE funds obtained from the Department of Science and Technology, Government of India. We acknowledge Dr. V.R. Sashidhar for estimating cytokinin in our samples using antibodies available in his laboratory.

Conflict of interest

There is no conflict of interest for the manuscript entitled “Light-Hormone Interaction in the Red Light Induced Suppression of Photomorphogenesis in Rice Seedlings” submitted to Protoplasma.

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  1. School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

    Ansuman Roy & Baishnab C. Tripathy

  2. Department of Botany, University of Delhi, New Delhi, India

    Dinabandhu Sahoo

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  1. Ansuman Roy
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Correspondence to Baishnab C. Tripathy.

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Roy, A., Sahoo, D. & Tripathy, B.C. Light-hormone interaction in the red-light-induced suppression of photomorphogenesis in rice seedlings. Protoplasma 253, 393–402 (2016). https://doi.org/10.1007/s00709-015-0818-1

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