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Genome sequence of bubaline alphaherpesvirus 1 (BuHV1) isolated in Australia in 1972

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Abstract

Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water buffalo (Bubalus bubalis) in 1972 in Australia. The virus was multiplied in MDBK cells, and the DNA was extracted and subjected to high-throughput sequencing. The reads were aligned and combined into a single genome sequence, with bovine alphaherpesvirus 5 (BoHV5) strain SV507/99 (accession number NC005261) as a reference. The BuHV1-b6 genome is a linear double-stranded DNA molecule, 137,452 bp long, with a GC content of 76.8%. The genome consists of two unique sequences: a long, or UL, sequence (103,818 bp) and a short, or US, sequence (9,586 bp), with the latter being flanked by inverted IR and TR elements of 12,024 bp each. The arrangement is typical of herpesvirus genomes of the D-type. The overall sequence has a 92.2% similarity at the nucleotide level to the reference BoHV5 strain. Our report provides a significant landmark in the history of herpesviruses, represented by the genome sequence of this 44-year-old virus isolate.

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Acknowledgements

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, project number 475802/2013-6), and Financiadora de Estudos e Projetos (FINEP, contract 01.10.0783.00). PMR, FRS and ACF are CNPq research follows. This work was performed as part of the doctoral studies of CMS (Programa de Pós-graduação em Ciências Veterinárias, UFRGS).

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Authors and Affiliations

  1. Institute of Veterinary Research “Desidério Finamor” (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil

    Camila Mengue Scheffer, Ana Paula Muterle Varela, Samuel Paulo Cibulski, Candice Schmidt, Willian Pinto Paim, Márcia Regina Loiko, Caroline Tochetto, Diane Alves de Lima, Cristine Cerva, Fabiana Quoos Mayer, Sylio Alfredo Petzhold & Paulo Michel Roehe

  2. Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil

    Camila Mengue Scheffer, Ana Paula Muterle Varela, Samuel Paulo Cibulski, Candice Schmidt, Fabrício Souza Campos, Willian Pinto Paim, Raíssa Nunes dos Santos, Thais Fumaco Teixeira, Márcia Regina Loiko, Caroline Tochetto, Helton Fernandes dos Santos, Diane Alves de Lima, Cristine Cerva, Ana Cláudia Franco & Paulo Michel Roehe

  3. Unit 44 (formerly with CSIRO Australia), 260 Cliveden Avenue, Corinda, QLD, 4075, Australia

    Toby St George

  4. Institute of Health Sciences, Feevale University, Rodovia RS-239, Novo Hamburgo, Rio Grande do Sul, CEP 93525075, Brazil

    Fernando Rosado Spilki

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  1. Camila Mengue Scheffer
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Correspondence to Camila Mengue Scheffer.

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Scheffer, C.M., Varela, A.P.M., Cibulski, S.P. et al. Genome sequence of bubaline alphaherpesvirus 1 (BuHV1) isolated in Australia in 1972. Arch Virol 162, 1169–1176 (2017). https://doi.org/10.1007/s00705-016-3218-8

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