Abstract
The present study was conducted to analyze the phylogenetic status of the genus Onobrychis and to evaluate the monophyly of its subgenera and sections and relationship among them. We sequenced the nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) and three chloroplast regions trnL-F, rpl32/rpl32-trnL(UAG) and ndhF-rlp32 for phylogenetic reconstruction of 51 species of Onobrychis. In all of our analyses, Eversmannia subspinosa, Corethrodendron scoparium, Greuteria membranacea and G. argyrea were chosen as outgroups. Phylogenetic analyses were performed by maximum parsimony, maximum likelihood and Bayesian methods. Our molecular data indicate that Onobrychis is monophyletic and composed of two main clades, each corresponding to the redefined subgenus Onobrychis (including sections Onobrychis and Hemicyclobrychis) and subgenus Sisyrosema (including sections Afghanicae, Laxiflorae, Heliobrychis, Hymenobrychis, Insignes, Lipskyanae and Litvinovianae), respectively. Sections Lipskyanae and Litvinovianae are newly established and described, representing distinct lineages within the genus. Onobrychis splendida, a species hitherto without a sectional position, along with some members of sect. Anthyllium were retrieved representatives of section Lipskyanae. Sections Afghanicae, Insignes, Heliobrychis and Hymenobrychis (with the inclusion of two species of section Anthyllium) are monophyletic. Sections Dendrobrychis and Lophobrychis are reduced to synonymy of section Onobrychis and Anthyllium to synonymy of section Hymenobrychis. A taxonomic treatment for the genus is presented.
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Acknowledgments
The present study was financially supported in part by Grant-in-Aids for Scientific Research, No. 89002433, to S.K. O (corresponding author) from INSF (Iran National Science Foundation). We would like to thank the staff of the herbaria of, FUMH, HKNRRC, HQNRRC, MSB, TARI and TUH to allow studying herbarium specimens and providing leaf materials. We also thank H. Zare for editing and improving linguistic of the text. The trees resulting from Parsimony, Likelihood and Bayesian analyses of nrDNA ITS and plastid datasets, trnL–F, rpl32/rpl32-trnL(UAG) and ndhF-rpl32 are presented as Electronic Supplementary Material in Online resource 1, 2, 3, 4. The aligned data matrix used in this study is presented as Online Resource 5.
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Online Resource 1 Fifty percent majority rule consensus tree resulting from Bayesian inference of the nrDNA ITS dataset. Numbers above branches are posterior probability and likelihood as well as parsimony bootstrap values, respectively. Values<50 % were not shown (PDF 132 kb)
606_2016_1343_MOESM2_ESM.pdf
Online Resource 2 Fifty percent majority rule consensus tree resulting from Bayesian inference of the plastid trnL-F dataset. Numbers above branches are posterior probability and likelihood as well as parsimony bootstrap values, respectively. Values<50 % were not shown (PDF 129 kb)
606_2016_1343_MOESM3_ESM.pdf
Online Resource 3 Fifty percent majority rule consensus tree resulting from Bayesian inference of the plastid rpl32/rpl32- trnLUAG dataset. Numbers above branches are posterior probability and likelihood as well as parsimony bootstrap values, respectively. Values<50 % were not shown (PDF 129 kb)
606_2016_1343_MOESM4_ESM.pdf
Online Resource 4 Fifty percent majority rule consensus tree resulting from Bayesian inference of the plastid ndhF-rpl32 dataset. Numbers above branches are posterior probability and likelihood as well as parsimony bootstrap values, respectively. Values<50 % were not shown (PDF 132 kb)
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Online Resource 1. Fifty percent majority rule consensus tree resulting from Bayesian inference of the nrDNA ITS dataset.
Online Resource 2. Fifty percent majority rule consensus tree resulting from Bayesian inference of the plastid trnL-F dataset.
Online Resource 3. Fifty percent majority rule consensus tree resulting from Bayesian inference of the plastid rpl32/rpl32- trnLUAG dataset.
Online Resource 4. Fifty percent majority rule consensus tree resulting from Bayesian inference of the plastid ndhF-rpl32 dataset.
Online Resource 5. The aligned data matrix used in this study.
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Amirahmadi, A., Kazempour-Osaloo, S., Kaveh, A. et al. The phylogeny and new classification of the genus Onobrychis (Fabaceae-Hedysareae): evidence from molecular data. Plant Syst Evol 302, 1445–1456 (2016). https://doi.org/10.1007/s00606-016-1343-1
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DOI: https://doi.org/10.1007/s00606-016-1343-1