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Binder-free and efficient voltammetric sensor based on Zn-Ca2CuO3 nanoparticles for simultaneous determination of amlodipine, acetaminophen, and ascorbic acid in hypertension patients

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Abstract

Amlodipine (AM) is a long active calcium channel blocker used to relax blood vessels by preventing calcium ion transport into the vascular walls and its supporting molecules acetaminophen (AP) and ascorbic acid (AA) are recommended for hypertension control and prevention. Considering their therapeutic importance and potential side effects due to over dosage, we have fabricated a sensor for individual and simultaneous determination of AA, AP, and AM in pharmaceuticals and human urine using novel Zn-doped Ca2CuO3 nanoparticles modified glassy carbon electrode (GCE). Optimally doped Ca2CuO3 (2.5 wt% Zn at Cu site) enhanced the detection of target molecules over much wider concentration ranges of 50 to 3130 µM for AA, 0.25 to 417 µM for AP, and 0.8 to 354 µM for AM with the corresponding lowest detection limits of 14 µM, 0.05 µM, and 0.07 µM, respectively. Furthermore, the Zn-Ca2CuO3/GCE exhibited excellent selectivity and high sensitivity even in the presence of several potential interfering agents. The usefulness of the developed electrode was tested using an amlodipine besylate tablet and urine samples of seven hypertension patients under medication. The results confirmed the presence of a significant amount of AP and AM in six patients’ urine samples indicating that the personalized medication is essential and the quantum of medication need to be fixed by knowing the excess medicines excreted through urine. Thus, the Zn-Ca2CuO3/GCE with a high recovery percentage and good sensitivity shall be useful in the pharmaceutical and biomedical sectors.

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Funding

The authors acknowledge DST sponsored Indo-Sri Lanka project (DST/INT/SL/P-30/2021) and MHRD-RUSA 2.0 (No. F.24–51/2014-U, Policy (TN-Multi-Gen), dated: 09.10.2018) for financial assistance. The authors also thank Mr. S. Arunachalam for many stimulating discussions.

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  1. Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi, 630003, Tamil Nadu, India

    G. Veerapandi & C. Sekar

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  1. G. Veerapandi
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Correspondence to C. Sekar.

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Urine samples for this research have been collected from the Government Hospital in Karaikudi, where doctors conduct clinical tests that meet ethical norms and protect participants’ rights and well-being. As a result, ethical approval may not always be necessary for the use of human urine samples.

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Veerapandi, G., Sekar, C. Binder-free and efficient voltammetric sensor based on Zn-Ca2CuO3 nanoparticles for simultaneous determination of amlodipine, acetaminophen, and ascorbic acid in hypertension patients. Microchim Acta 191, 409 (2024). https://doi.org/10.1007/s00604-024-06473-3

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