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Computer-aided design of molecularly imprinted polymer reinforced by double hybrid monomers for selective purification of hydroxycamptothecin

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Abstract

A kind of hydroxycamptothecin (HCPT) hybrid molecularly imprinted polymer (AT/MA-HMIPs) with high selectivity and hard silicon skeleton was successfully prepared based on double hybrid monomers. The relationship between templates and functional monomers was studied through computer molecular simulation and experiments. Three single-monomer molecularly imprinted polymers were prepared as controls. The Langmuir isotherm and pseudo-second-order kinetic models were found to fit well with the adsorption results. The maximum adsorption capacity was 18.79 mg/g, and equilibrium was reached within 20 min. Moreover, it shows excellent selectivity (imprinting factor is 10.73) and good recoverability (after 10 adsorption-desorption cycles, the adsorption capacity only decreases by 7.75%) for HCPT. The purity of HCPT can reach 80.86% after being put into a solid phase extraction column and used in an actual sample, and the yield was 61.43%. This study lays the fundament for the development of excellent HCPT molecularly imprinted composites.

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Acknowledgements

The National Key Research and Development Project of China (2022YFD2200805), the Fundamental Research Fund for Central Universities (2572022DP06), Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team), and the 111 Project, China (B20088).

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Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China

    Fang Nie, Chunying Li, Bin Qiao, Junling Wang, Yuan Gao & Chunjian Zhao

  2. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China

    Fang Nie, Chunying Li, Bin Qiao, Junling Wang, Yuan Gao & Chunjian Zhao

  3. Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin, 150040, China

    Fang Nie, Chunying Li, Bin Qiao, Junling Wang, Yuan Gao & Chunjian Zhao

  4. Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Harbin, 150040, China

    Fang Nie, Chunying Li, Bin Qiao, Junling Wang, Yuan Gao & Chunjian Zhao

  5. Hisun Pharmaceutical (Hangzhou) Co., Ltd., No 1, Road, Xukou Town, Fuyang District, Hangzhou, 311404, China

    Jie Liu

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  1. Fang Nie
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Nie, F., Li, C., Qiao, B. et al. Computer-aided design of molecularly imprinted polymer reinforced by double hybrid monomers for selective purification of hydroxycamptothecin. Microchim Acta 190, 419 (2023). https://doi.org/10.1007/s00604-023-05997-4

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