SpringerLink
Log in

An electrochemical aptasensor for detection of streptomycin based on signal amplification assisted by functionalized gold nanoparticles and hybridization chain reaction

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A ratiometric electrochemical aptasensor based on gold nanoparticles (AuNPs) functionalization and hybridization chain reaction (HCR) assisted signal amplification has been for the first time designed for the detection of streptomycin (STR). The double-stranded DNA (dsDNA) formed by the hybridization of ferrocene (Fc)-labeled STR aptamer (Apt) and capture probe (CP) is first immobilized on the gold electrode (GE) surface via Au–S reaction. The specific binding of the target and Apt results in numerous Fc detachment from the sensing interface. Then, the remaining single-stranded CP is combined with AuNPs modified with initiator DNA (iDNA) by auxiliary DNA (aDNA). Among them, the iDNA triggers HCR between two hairpin probes (H1/H2), thus capturing a large number of methylene blue (MB) electrochemical probe, which generates a strong electrochemical signal of MB and a weak electrochemical signal of Fc. Signals are collected by square wave voltammetry (the potential window ranging from -0.5 V to 0.6 V, vs. Ag/AgCl ), and the oxidation peak currents at -0.200 V (MB) and 0.416 V (Fc) are recorded. The use of the ratiometric method has effectively improved the accuracy and reliability of the analysis. The successful application of AuNPs and HCR greatly improves the sensitivity of the sensor, and the detection limit is as low as 0.08 pM. It can sensitively determine STR in the range 0.1 pM to 10 nM. In addition, the designed aptasensor has been successfully applied to the detection of STR in milk and honey samples.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1 
Fig. 2 
Fig. 3

Similar content being viewed by others

References

  1. Schatz A, Bugie E, Waksman SA (2005) Streptomycin, a substance exhibiting antibiotic activity against gram-positive and gram-negative bacteria. 1944. Clin Orthop Relat Res 437:3–6

    Article  Google Scholar 

  2. Becker B, Cooper MA (2013) Aminoglycoside antibiotics in the 21st century. ACS Chem Biol 8:105–115

    Article  CAS  PubMed  Google Scholar 

  3. Knecht BG, Strasser A, Dietrich R, Märtlbauer E, Niessner R, Weller MG (2004) Automated microarray system for the simultaneous detection of antibiotics in milk. Anal Chem 76:646–654

    Article  CAS  PubMed  Google Scholar 

  4. Du BY, Wen F, Zhang YD, Zheng N, Li SL, Li FD, Wang JQ (2019) Presence of tetracyclines, quinolones, lincomycin and streptomycin in milk. Food Control 100:171–175

    Article  CAS  Google Scholar 

  5. Conzuelo F, Gamella M, Campuzano S, Pinacho DG, Reviejo AJ, Marco MP, **arrón JM (2012) Disposable and integrated amperometric immunosensor for direct determination of sulfonamide antibiotics in milk. Biosens Bioelectron 36:81–88

    Article  CAS  PubMed  Google Scholar 

  6. Granados O, Meza G (2007) A direct HPLC method to estimate streptomycin and its putative ototoxic derivative, streptidine, in blood serum: application to streptomycin-treated humans. J Pharm Biomed Anal 43:625–630

    Article  CAS  PubMed  Google Scholar 

  7. Smadi N, Jammoul A, Darra NE (2019) Assessment of Antibiotic and Pesticides Residues in Breast Milk of Syrian Refugee Lactating Mothers. Toxics 7:39

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Zhu WX, Yang JZ, Wei W, Liu YF, Zhang SS (2008) Simultaneous determination of 13 aminoglycoside residues in foods of animal origin by liquid chromatography-electrospray ionization tandem mass spectrometry with two consecutive solid-phase extraction steps. J Chromatogr A 1207:29–37

    Article  CAS  PubMed  Google Scholar 

  9. Stubbings G, Bigwood T (2009) The development and validation of a multiclass liquid chromatography tandem mass spectrometry (LC-MS/MS) procedure for the determination of veterinary drug residues in animal tissue using a QuEChERS (QUick, Easy, CHeap, Effective, Rugged and Safe) approach. Anal Chim Acta 637:68–78

    Article  CAS  PubMed  Google Scholar 

  10. Guidi LR, Santos FA, Ribeiro AC, Fernandes C, Silva LH, Gloria MB (2017) A simple, fast and sensitive screening LC-ESI-MS/MS method for antibiotics in fish. Talanta 163:85–93

    Article  CAS  PubMed  Google Scholar 

  11. Eickner T, Reske T, Illner S, Schmitz K-P, Martin DP, Grabow N (2017) Fast parallel quantification of dual drug systems via UV-VIS spectrometry – a case study with antibiotic substances, Current Directions in Biomedical Engineering. Curr Dir Biomed 3:375–378

    Google Scholar 

  12. Wang S, Zhang HY, Wang L, Duan ZJ, Kennedy I (2006) Analysis of sulphonamide residues in edible animal products: a review. Food Addit Contam 23:362–384

    Article  CAS  PubMed  Google Scholar 

  13. Mishra GK, Sharma A, Bhand S (2015) Ultrasensitive detection of streptomycin using flow injection analysis-electrochemical quartz crystal nanobalance (FIA-EQCN) biosensor. Biosens Bioelectron 67:532–539

    Article  CAS  PubMed  Google Scholar 

  14. You F, Wei J, Cheng Y, Wen Z, Ding C, Hao N, Wang K (2021) Selective and sensitive photoelectrochemical aptasensor for streptomycin detection based on Bi(4)VO(8)Br/Ti(3)C(2) nanohybrids. J Hazard Mater 414:125539

    Article  CAS  PubMed  Google Scholar 

  15. Yang LQ, Zhao J, Wang CJ, Wang Z, **ng CJ, Guo H, Wang YM, Zhao ZJ, Hu ZJ, Cai ZY (2021) Bi/BiVO(4)/NiFe-LDH heterostructures with enhanced photoelectrochemical performance for streptomycin detection. J Environ Sci (China) 109:114–122

    Article  CAS  PubMed  Google Scholar 

  16. Soheili V, Taghdisi SM, HassanzadehKhayyat M, FazlyBazzaz BS, Ramezani M, Abnous K (2016) Colorimetric and ratiometric aggregation assay for streptomycin using gold nanoparticles and a new and highly specific aptamer. Microchim Acta 183:1687–1697

    Article  CAS  Google Scholar 

  17. Wang Z, Sun Y, Liang D, Zeng Y, He S, Mari GM, Peng T, Jiang H (2020) Highly sensitive chromatographic time-resolved fluoroimmunoassay for rapid onsite detection of streptomycin in milk. J Dairy Sci 103:8750–8760

    Article  CAS  PubMed  Google Scholar 

  18. Zhu Q, Liu L, Wang R, Zhou X (2021) A split aptamer (SPA)-based sandwich-type biosensor for facile and rapid detection of streptomycin. J Hazard Mater 403:123941

    Article  CAS  PubMed  Google Scholar 

  19. Roushani M, Ghanbari K (2019) An electrochemical aptasensor for streptomycin based on covalent attachment of the aptamer onto a mesoporous silica thin film-coated gold electrode. Microchim Acta 186:115

    Article  Google Scholar 

  20. Yin J, Guo W, Qin X, Pei M, Wang L, Ding F (2016) A regular “signal attenuation” electrochemical aptasensor for highly sensitive detection of streptomycin. New J Chem 40:9711–9718

    Article  CAS  Google Scholar 

  21. Zarei K, Ghorbani M (2019) Fabrication of a new ultrasensitive AuNPs-MIC-based sensor for electrochemical determination of streptomycin. Electrochim Acta 299:330–338

    Article  CAS  Google Scholar 

  22. Yuan YH, Chi BZ, Wen SH, Liang RP, Li ZM, Qiu JD (2018) Ratiometric electrochemical assay for sensitive detecting microRNA based on dual-amplification mechanism of duplex-specific nuclease and hybridization chain reaction. Biosens Bioelectron 102:211–216

    Article  CAS  PubMed  Google Scholar 

  23. Zhao YX, Chen F, Li Q, Wang LH, Fan CH (2015) Isothermal Amplification of Nucleic Acids. Chem Rev 115:12491–12545

    Article  CAS  PubMed  Google Scholar 

  24. Liao X, Zhang C, Machuki JO, Wen X, Chen D, Tang Q, Gao F (2021) Proximity hybridization triggered hybridization chain reaction for label-free electrochemical homogeneous aptasensors. Talanta 226:122058

    Article  CAS  PubMed  Google Scholar 

  25. Bi S, Yue SZ, Zhang SS (2017) Hybridization chain reaction: a versatile molecular tool for biosensing, bioimaging, and biomedicine. Chem Soc Rev 46:4281–4298

    Article  CAS  PubMed  Google Scholar 

  26. Huang ZM, He DG, Li HW (2020) A fluorometric assay of thrombin using magnetic nanoparticles and enzyme-free hybridization chain reaction. Microchim Acta 187:295

    Article  CAS  Google Scholar 

  27. Lv MM, Fan SF, Wang QL, Lv QY, Song XJ, Cui HF (2019) An enzyme-free electrochemical sandwich DNA assay based on the use of hybridization chain reaction and gold nanoparticles: application to the determination of the DNA of Helicobacter pylori. Microchim Acta 187:73

    Article  Google Scholar 

  28. Miti A, Thamm S, Müller P, Csáki A, Fritzsche W, Zuccheri G (2020) A miRNA biosensor based on localized surface plasmon resonance enhanced by surface-bound hybridization chain reaction. Biosens Bioelectron 167:112465

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Liu D, Meng SY, Shen XL, Li YY, Yan XH, You TY (2021) Dual-ratiometric aptasensor for streptomycin detection based on the in-situ coupling of photoelectrochemical and electrochemical assay with a bifunctional probe of methylene blue. Sens Actuators B Chem 129529:l332

    Google Scholar 

  30. Zhang CY, Chen J, Sun R, Huang ZJ, Luo ZW, Zhou C, Wu MF, Duan YX, Li YX (2020) The Recent Development of Hybridization Chain Reaction Strategies in Biosensors. ACS Sens 5:2977–3000

    Article  CAS  PubMed  Google Scholar 

  31. Zhang QY (2022) Application of Hybridization Chain Reaction (HCR) in Electrochemical Analysis. Int J Electrochem Sci 17 Article Number:220227, doi:10.20964/2022.02.14

  32. Hai-hu Y, De-sheng J (2002) Gold nanoparticulate thin films fabricated by the electrostatic self-assembly process. J Wuhan Univ Technol Mater 17:38–41

    Article  Google Scholar 

  33. Haishuai P, Yuanyuan H, Lei Z, Fuxin Z, Yufang L, Jianbin Z, Rong J, Yuxuan S, Bini W (2022) A novel electrochemical aptasensor based on Ti3C2-MOFs nanocomposites for rapid streptomycin detection in milk samples. Sens Actuators B Chem 368:132119

    Article  Google Scholar 

  34. Jahanbani S, Benvidi A (2016) Comparison of two fabricated aptasensors based on modified carbon paste/oleic acid and magnetic bar carbon paste/Fe3O4@oleic acid nanoparticle electrodes for tetracycline detection. Biosens Bioelectron 85:553–562

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (21475024) and the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_15R11), China.

Author information

Authors and Affiliations

  1. Key Laboratory for Analytical Science of Food Safety and Biology, MOE, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350108, Fujian, China

    Zhoubing Zhang, **aorun Jia & Xueqin Xu

Authors
  1. Zhoubing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. **aorun Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xueqin Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xueqin Xu.

Ethics declarations

Compliance with ethical standards

The authors declare that they have no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 6915 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Z., Jia, X. & Xu, X. An electrochemical aptasensor for detection of streptomycin based on signal amplification assisted by functionalized gold nanoparticles and hybridization chain reaction. Microchim Acta 190, 152 (2023). https://doi.org/10.1007/s00604-023-05737-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-023-05737-8

Keywords

Search

Navigation