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Molecularly imprinted bulk and solgel optosensing based on biomass carbon dots derived from watermelon peel for detection of ethyl carbamate in alcoholic beverages

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Abstract

Biomass carbon dots synthesized by biological waste conform to the trend of ecological environmental protection and the requirements of green chemistry, which show great application potential in practice. In the study, we used watermelon peels as the raw materials to synthesize a novel blue biomass carbon dots (CDs) by a hydrothermal process with high fluorescence quantum yield of 22.8%. Through bulk polymerization and solgel method, two kinds of core–shell nanospheres were developed as fluorescent probes to recognize and detect ethyl carbamate (EC) rapidly without complex samples pretreatment. The obtained CDs@MIPs integrated the high-performance optical characteristics of CDs with excellent selectivity and adsorption of MIPs, which showed ideal linear relationships in the EC concentration range 1–120 μg L−1 and low LOD of 0.57 μg L−1 and 0.94 μg L−1, respectively. Both CDs@MIPs have a short equilibration time which was around 20 min, and the imprinting factors (IF) are 4.04 and 2.62. The recoveries of the six spiked samples were satisfying, and the RSD precisions were lower than 5.57%. Gas chromatography–mass spectrometry was seen as a parallel analysis to validate the correctness of the results, which indicated the practicability and reliability of the developed method. This proposal strategy of optical sensors provided an effective channel for trace EC recognition, with numerous advantages, involving eco-friendly, low cost, high sensitivity, separation effect, and good selectivity.

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References

  1. Liu HL, Sun BG (2018) Effect of fermentation processing on the flavor of Baijiu. J Agric Food Chem 66:5425–5432. https://doi.org/10.1021/acs.jafc.8b00692

    Article  CAS  PubMed  Google Scholar 

  2. Baan R, Straif K, Grosse Y, Secretan B, El Ghissassi F, Bouvard V, Altieri A, Cogliano V (2007) Carcinogenicity of alcoholic beverages. Lancet Oncol 8(4):292–293. https://doi.org/10.1016/S1470-2045(07)70099-2

    Article  PubMed  Google Scholar 

  3. Hasnip S, Crews C, Potter N, Christy J, Chan D, Bondu T, Matthews W, Walters B, Patel K (2007) Survey of ethyl carbamate in fermented foods sold in the United Kingdom in 2004. J Agric Food Chem 55(7):2755–2759. https://doi.org/10.1021/jf063121c

    Article  CAS  PubMed  Google Scholar 

  4. Zhao XR, Du GC, Zou HJ, Fu JW, Zhou JW, Chen J (2013) Progress in preventing the accumulation of ethyl carbamate in alcoholic beverages. Trends Food Sci Tech 32(2):97–107. https://doi.org/10.1016/j.tifs.2013.05.009

    Article  CAS  Google Scholar 

  5. Wang C, Wang M, Zhang MP (2021) Ethyl carbamate in Chinese liquor (Baijiu): presence, analysis, formation, and control. Appl Microbiol Biot 105:4383–4395. https://doi.org/10.1007/s00253-021-11348-1

    Article  CAS  Google Scholar 

  6. Chen DW, Ren YP, Zhong QD, Shao Y, Zhao YF, Wu YN (2017) Ethyl carbamate in alcoholic beverages from China: levels, dietary intake, and risk assessment. Food Control 72:283–288. https://doi.org/10.1016/j.foodcont.2015.10.047

    Article  CAS  Google Scholar 

  7. Jiao ZH, Dong YC, Chen QH (2014) Ethyl carbamate in fermented beverages: presence, analytical chemistry, formation mechanism, and mitigation proposals. Compr Rev Food Sci F 13(4):611–626. https://doi.org/10.1111/1541-4337.12084

    Article  CAS  Google Scholar 

  8. Nóbrega I, Pereira GE, Silva M, Pereira E, Medeiros MM, Telles DL, Albuquerque EC, Oliveira JB, Lachenmeier DW (2015) Improved sample preparation for GC–MS–SIM analysis of ethyl carbamate in wine. Food Chem 177:23–28. https://doi.org/10.1016/j.foodchem.2014.12.031

    Article  CAS  PubMed  Google Scholar 

  9. Zhao XR, Jiang CX (2015) Determination of ethyl carbamate in fermented liquids by ultra high performance liquid chromatography coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometer. Food Chem 177:66–71. https://doi.org/10.1016/j.foodchem.2015.01.025

    Article  CAS  PubMed  Google Scholar 

  10. Luo L, Lei HT, Yang JY, Liu GL, Sun YM, Bai WD, Wang H, Shen YD, Chen S, Xu ZL (2017) Development of an indirect ELISA for the determination of ethyl carbamate in Chinese rice wine. Anal Chim Acta 950(15):162–169. https://doi.org/10.1016/j.aca.2016.11.008

    Article  CAS  PubMed  Google Scholar 

  11. **a Q, Yang CJ, Wu CD, Zhou RQ, Li YF (2018) Quantitative strategies for detecting different levels of ethyl carbamate (EC) in various fermented food matrices: An overview. Food Control 84:499–512. https://doi.org/10.1016/j.foodcont.2017.09.008

    Article  CAS  Google Scholar 

  12. Yan X, Li HX, Sun XG (2018) Review of optical sensors for pesticides. Trends Analyt Chem 103:1–20. https://doi.org/10.1016/j.trac.2018.03.004

    Article  CAS  Google Scholar 

  13. Yuan XY, Zhang DW, Zhu XC, Liu HL, Sun BG (2021) Triple-dimensional spectroscopy combined with chemometrics for the discrimination of pesticide residues based on ionic liquid-stabilized Mn-ZnS quantum dots and covalent organic frameworks. Food Chem 342(16):128299. https://doi.org/10.1016/j.foodchem.2020.128299

    Article  CAS  PubMed  Google Scholar 

  14. Zhang Y, Zhu XC, Li MJ, Liu HL, Sun BG (2022) Temperature-responsive covalent organic framework-encapsulated carbon dot-based sensing platform for pyrethroid detection via fluorescence response and smartphone readout. J Agric Food Chem 70(20):6059–6071. https://doi.org/10.1021/acs.jafc.2c01568

    Article  CAS  PubMed  Google Scholar 

  15. Zhao Y, Liu HL, Sun BG (2022) Chiral induction in carbazole-conjugated covalent organic frameworks: a supersensitive fluorescence sensing platform for chiral recognition. Sens Actuators B Chem 354:131253. https://doi.org/10.1016/j.snb.2021.131253

    Article  CAS  Google Scholar 

  16. Sun XC, Lei Y (2017) Fluorescent carbon dots and their sensing applications. Trends Analyt Chem 89:163–180. https://doi.org/10.1016/j.trac.2017.02.001

    Article  CAS  Google Scholar 

  17. Yuan XY, Jiang W, Wang J, Liu HL, Sun BG (2020) High-performance multiporous imprinted microspheres based on N-doped carbon dots exfoliated from covalent organic framework for flonicamid optosensing. ACS Appl Mater Interfaces 12(22):25150–25158. https://doi.org/10.1021/acsami.0c04766

    Article  CAS  PubMed  Google Scholar 

  18. Peng ZL, Han X, Li SH, Al-Youbi AO, Bashammakh AS, El-Shahawi MS, Leblanc RM (2017) Carbon dots: Biomacromolecule interaction, bioimaging and nanomedicine. Coord Chem Rev 343(15):256–277. https://doi.org/10.1016/j.ccr.2017.06.001

    Article  CAS  Google Scholar 

  19. Baker SN, Baker GA (2010) Luminescent carbon nanodots: emergent nanolights. Angew Chem Int Ed 49(38):6726–6744. https://doi.org/10.1002/anie.200906623

    Article  CAS  Google Scholar 

  20. Zhu XC, Yuan XY, Han LX, Liu HL, Sun BG (2021) A smartphone-integrated optosensing platform based on red-emission carbon dots for real-time detection of pyrethroids. Biosens Bioelectron 191(1):113460. https://doi.org/10.1016/j.bios.2021.113460

    Article  CAS  PubMed  Google Scholar 

  21. Liu HC, Ding J, Zhang K, Ding L (2019) Construction of biomass carbon dots based fluorescence sensors and their applications in chemical and biological analysis. Trends Analyt Chem 118:315–337. https://doi.org/10.1016/j.trac.2019.05.051

    Article  CAS  Google Scholar 

  22. Huang JT, Liu JJ, Wang J (2020) Optical properties of biomass-derived nanomaterials for sensing catalytic biomedical and environmental applications. Trends Analyt Chem 124:155800. https://doi.org/10.1016/j.trac.2019.115800

    Article  CAS  Google Scholar 

  23. Wang CJ, Shi HX, Yang M, Yan YJ, Liu EZ, Ji Z, Fan J (2020) Facile synthesis of novel carbon quantum dots from biomass waste for highly sensitive detection of iron ions. Mater Res Bull 124:110730–110731. https://doi.org/10.1016/j.materresbull.2019.110730

    Article  CAS  Google Scholar 

  24. Jiang W, Zhao Y, Zhang DW, Zhu XC, Liu HL, Sun BG (2021) Efficient and robust dual modes of fluorescence sensing and smartphone readout for the detection of pyrethroids using artificial receptors bound inside a covalent organic framework. Biosens Bioelectron 194:113582. https://doi.org/10.1016/j.bios.2021.113582

    Article  CAS  PubMed  Google Scholar 

  25. Shirani MP, Rezaei B, Ensafi AA, Ramezani M (2021) Development of an eco-friendly fluorescence nanosensor based on molecularly imprinted polymer on silica-carbon quantum dot for the rapid indoxacarb detection. Food Chem 339:127920. https://doi.org/10.1016/j.foodchem.2020.127920S

    Article  CAS  PubMed  Google Scholar 

  26. Liu HC, Ding L, Chen LG, Chen YH, Zhou TY, Li HY, Xu Y, Zhao L, Huang N (2018) A facile, green synthesis of biomass carbon dots coupled with molecularly imprinted polymers for highly selective detection of oxytetracycline. J Ind Eng Chem 69:455–463. https://doi.org/10.1016/j.jiec.2018.10.007

    Article  CAS  Google Scholar 

  27. Han LX, Meng C, Zhang DW, Liu HL, Sun BG (2022) Fabrication of a fluorescence probe via molecularly imprinted polymers on carbazole-based covalent organic frameworks for optosensing of ethyl carbamate in fermented alcoholic beverages. Anal Chim Acta 1192:339381. https://doi.org/10.1016/j.aca.2021.339381

    Article  CAS  PubMed  Google Scholar 

  28. Lu MC, Duan YX, Song YH, Tan JS, Zhou L (2018) Green preparation of versatile nitrogen-doped carbon quantum dots from watermelon juice for cell imaging, detection of Fe3+ ions and cysteine, and optical thermometry. J Mol Liq 269:66–774. https://doi.org/10.1016/j.molliq.2018.08.101

    Article  CAS  Google Scholar 

  29. Zhu XC, Han LX, Liu HL, Sun BG (2022) A smartphone-based ratiometric fluorescent sensing system for on-site detection of pyrethroids by using blue-green dual-emission carbon dots. Food Chem 379:132154. https://doi.org/10.1016/j.foodchem.2022.132154

    Article  CAS  PubMed  Google Scholar 

  30. Thangaraj B, Solomon PR, Ranganathan S (2019) Synthesis of carbon quantum dots with special reference to biomass as a source -a review. Curr Pharm Design 25:1455–1476. https://doi.org/10.2174/1381612825666190618154518

    Article  CAS  Google Scholar 

  31. Liu HC, Ding J, Zhang K, Ding L (2020) Fabrication of carbon dots@restricted access molecularly imprinted polymers for selective detection of metronidazole in serum. Talanta 209:120508. https://doi.org/10.1016/j.talanta.2019.120508

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31822040, 32072335), the National Key R&D Program of China (No. 2018YFC1602300), and the Graduate Research Ability Promotion Project of Bei**g Technology and Business University in 2022.

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  1. Bei**g Technology and Business University, 11 Fucheng Road, Bei**g, 100048, China

    Luxuan Han, Pei Zhu, Huilin Liu & Baoguo Sun

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  1. Luxuan Han
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  3. Huilin Liu
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  4. Baoguo Sun
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Correspondence to Huilin Liu.

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Han, L., Zhu, P., Liu, H. et al. Molecularly imprinted bulk and solgel optosensing based on biomass carbon dots derived from watermelon peel for detection of ethyl carbamate in alcoholic beverages. Microchim Acta 189, 286 (2022). https://doi.org/10.1007/s00604-022-05388-1

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