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Colorimetric determination of cysteine by a paper-based assay system using aspartic acid modified gold nanoparticles

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Abstract

A method is described for cysteine (Cys) determination on paper-based analytical devices using aspartic acid modified gold nanoparticles (Asp-AuNPs). The Asp-AuNPs were characterized by their size, zeta potential, and UV-visible absorption spectrum. After the addition of Cys, it will interact with Asp-AuNPs selectively and leads to the aggregation of Asp-AuNPs. A color change from red to blue can be observed on the paper-based analytical devices. The results were recorded using a cell phone and subsequently analyzed using the Photoshop software. The ratiometric color intensity at red channel and blue channel (Red/Blue) increased linearly in the range 99.9–998.7 μM for Cys (R = 0.9984), and the limit of detection was 1.0 μM. The effects of assay conditions have been investigated and are discussed. The Cys concentration was determined as (0.27 ± 0.02 mM) in human plasma, and the recovery was from 99.2 to 101.1%.

Schematic representation of the paper-based assay system using aspartic acid modified gold nanoparticles (Asp-AuNPs). The ratiometric color intensity method was used for the cysteine (Cys) determination.

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Funding

This work was financially supported by the National Natural Science Foundation of China (81202492), the Service Local Foundation of Shaanxi Provincial Department of Education (19JC035), the Foundation of Science and Technology Department of Shaanxi Province of China (2020JM-614), and the Natural Science of Shaanxi Educational Committee of China (18JK0664, 18JK0665).

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Authors and Affiliations

  1. School of Pharmacy, **’ an Medical University, **’an, 710021, China

    Chunye Liu, Yanqing Miao, Xuejiao Zhang & **aojun Zhao

  2. Department of Medical Technology, **’ an Medical University, **’an, 710021, China

    Shuli Zhang

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  1. Chunye Liu
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  2. Yanqing Miao
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Correspondence to Chunye Liu.

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Liu, C., Miao, Y., Zhang, X. et al. Colorimetric determination of cysteine by a paper-based assay system using aspartic acid modified gold nanoparticles. Microchim Acta 187, 362 (2020). https://doi.org/10.1007/s00604-020-04333-4

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