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In vitro acaricidal activity of 1,8-cineole against Sarcoptes scabiei var. cuniculi and regulating effects on enzyme activity

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Abstract

1,8-Cineole found in many essential oils is a monoterpene and acts as a repellent against Sarcoptes scabiei var. cuniculi. In the present study, the acaricidal activity of 1,8-cineole against S. scabiei var. cuniculi was evaluated and the acaricidal mechanism was also investigated by assaying enzyme activities. The results showed that the lethal concentration of 50 % (LC50) value (95 % confidence limit (CL)) and the lethal time of 50 % (LT50) value (95 % CL) of 1,8-cineole were 2.77 mg/mL and 3.606 h, respectively. The pathological changes under transmission electron microscopy showed that the morphology of the mitochondria was abnormal, the cell nuclear membrane was damaged, and the nuclear chromatin was dissoluted. The activities of superoxide dismutase (SOD), glutathione-s-transferases (GSTs), monoamine oxidase (MAO), nitric oxide synthase (NOS), and acetylcholinesterase (AChE) were significantly changed after treatment with 1,8-cineole for 4, 8, 12, and 24 h. SOD and GSTs are associated with the protection mechanism of scabies mites. And, the activities of SOD and GSTs were increased as compared with the control group. MAO, AChE, and NOS are associated with the nervous system of scabies mites. The activity of MAO was increased whereas the AChE was suppressed. The activity of NOS was suppressed in the high-dose group whereas increased in the middle-dose group and low-dose group. These results indicated that the mechanism of 1,8-cineole mainly attributed to the changes of these enzyme activities related to the nervous system of scabies mites.

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Acknowledgments

This research was financially supported by National Natural Science Foundation of China (Grant No. 31272612, 31372477); the Doctoral Program of Higher Education Research Fund (Instructor Dr. Class 20105103110001); the Sichuan Youth Science and Technology Innovation Research Team for waterfowl disease prevention and control (2013TD0015); and the Sichuan International Cooperation Projects (2014HH0058).

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Authors and Affiliations

  1. Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

    Zhiqiang Hu, Zhenzhen Chen, Zhongqiong Yin, Xu Song, Li Li, Yuanfeng Zou, **aoxia Liang, Lixia Li, Changliang He, Lizi Yin, Cheng Lv, Ling Zhao, Gang Su, Gang Ye & Fei Shi

  2. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

    Renyong Jia

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  1. Zhiqiang Hu
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Correspondence to Zhongqiong Yin or Renyong Jia.

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Zhiqiang Hu, Zhenzhen Chen, and Zhongqiong Yin contribute equally to this work and should be considered as the first author.

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Hu, Z., Chen, Z., Yin, Z. et al. In vitro acaricidal activity of 1,8-cineole against Sarcoptes scabiei var. cuniculi and regulating effects on enzyme activity. Parasitol Res 114, 2959–2967 (2015). https://doi.org/10.1007/s00436-015-4498-8

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