Abstract
Untreated periodontal disease may influence general health. However, how may a physician, who is not trained in periodontal probing, detect untreated periodontitis? Activated matrix metalloproteinase-8 (aMMP-8) in saliva correlates with periodontal probing parameters. Thus, sensitivity and specificity of a chair-side test for aMMP-8 to detect periodontitis were evaluated. Thirty cases [untreated chronic periodontitis (ChP); 15 generalized moderate and 15 generalized severe] and 30 controls [probing depths (PD) ≤3 mm, vertical probing attachment level (PAL-V) ≤2 mm at <30 % of sites) were examined periodontally (PD, PAL-V, bleeding on probing). Subsequently, the aMMP-8 test was performed. The test kit becomes positive with ≥25 ng/ml aMMP-8 in the sample. The aMMP-8 test was positive in 87 % of ChP and in 40 % of controls. That corresponds to a sensitivity of 87 % and a specificity of 60 %. The sensitivity to detect generalized severe ChP was 93 % (60 % specificity). Backward stepwise logistic regression analysis to explain positive aMMP-8 tests identified exclusively ChP with an odds ratio of 9.8 (p < 0.001). Positive results of the aMMP-8 test significantly correlate with generalized ChP. The aMMP-8 test may be used by physicians to detect periodontitis in their patients.
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Acknowledgments
We wish to thank Dr. Almut von Schwedler, MBBS (Tas), Matcham, NSW, Australia, for her valuable and competent support in the preparation of this manuscript. This study was self-funded by the authors and their institutions. The PerioMarker® aMMP-8 test kits were provided by Chlorhexamed®, GlaxoSmithKline Consumer Healthcare GmbH & Co. KG, Bühl, and Hager & Werken GmbH & Co. KG, Duisburg, Germany.
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The authors declare that they have no financial or other relationships that might lead to a conflict of interest.
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ClinicalTrials.gov Identifier: NCT02280122.
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Izadi Borujeni, S., Mayer, M. & Eickholz, P. Activated matrix metalloproteinase-8 in saliva as diagnostic test for periodontal disease? A case–control study. Med Microbiol Immunol 204, 665–672 (2015). https://doi.org/10.1007/s00430-015-0413-2
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DOI: https://doi.org/10.1007/s00430-015-0413-2