Abstract
Main conclusion
A novel cytochrome P450 from Tripterygium wilfordii, CYP81AM1, specifically catalyses the C-15 hydroxylation of dehydroabietic acid. This is the first CYP450 to be found in plants with this function.
Abstract
Cytochrome P450 oxygenases (CYPs) play an important role in the post-modification in biosynthesis of plant bioactive terpenoids. Here, we found that CYP81AM1 can catalyze the formation of 15-hydroxydehydroabietic acid by in vitro enzymatic reactions and in vivo yeast feeding assays. This is the first study to show that CYP81 family enzymes are involved in the hydroxylation of abietane diterpenoids. At the same time, we found that CYP81AM1 could not catalyse abietatriene and dehydroabietinol, suggesting that the occurrence of the reaction may be related to the carboxyl group. Through molecular docking and site mutations, it was found that some amino acid sites (F104, K107) near the carboxyl group had an important effect on enzyme activity, also suggesting that the carboxyl group played an important role in the occurrence of the reaction.
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Data availability
The data supporting the findings of this study are available from the corresponding author Wei Gao, on reasonable request.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (81773830), the National Key R&D Program of China (2020YFA0908000), the Key Project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (2060302-1806-03), and the National Program for Special Support of Eminent Professionals.
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Communicated by Anastasios Melis.
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Wang, J., Su, P., Gao, L. et al. A cytochrome P450 CYP81AM1 from Tripterygium wilfordii catalyses the C-15 hydroxylation of dehydroabietic acid. Planta 254, 95 (2021). https://doi.org/10.1007/s00425-021-03743-9
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DOI: https://doi.org/10.1007/s00425-021-03743-9