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Differential proteomic analysis reveals sequential heat stress-responsive regulatory network in radish (Raphanus sativus L.) taproot

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Abstract

Main conclusion

Differential abundance protein species (DAPS) involved in reducing damage and enhancing thermotolerance in radish were firstly identified. Proteomic analysis and omics association analysis revealed a HS-responsive regulatory network in radish.

Heat stress (HS) is a major destructive factor influencing radish production and supply in summer, for radish is a cool season vegetable crop being susceptible to high temperature. In this study, the proteome changes of radish taproots under 40 °C treatment at 0 h (Control), 12 h (Heat12) and 24 h (Heat24) were analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification) approach. In total, 2258 DAPS representing 1542 differentially accumulated uniprotein species which respond to HS were identified. A total of 604, 910 and 744 DAPS was detected in comparison of Control vs. Heat12, Control vs. Heat24, and Heat12 vs. Heat24, respectively. Gene ontology and pathway analysis showed that annexin, ubiquitin-conjugating enzyme, ATP synthase, heat shock protein (HSP) and other stress-related proteins were predominately enriched in signal transduction, stress and defense pathways, photosynthesis and energy metabolic pathways, working cooperatively to reduce stress-induced damage in radish. Based on iTRAQ combined with the transcriptomics analysis, a schematic model of a sequential HS-responsive regulatory network was proposed. The initial sensing of HS occurred at the plasma membrane, and then key components of stress signal transduction triggered heat-responsive genes in the plant protective metabolism to re-establish homeostasis and enhance thermotolerance. These results provide new insights into characteristics of HS-responsive DAPS and facilitate dissecting the molecular mechanisms underlying heat tolerance in radish and other root crops.

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Abbreviations

DAPS:

Differential abundance protein species

DEG:

Differentially expressed gene

GO:

Gene ontology

HS:

Heat stress

HSP:

Heat shock protein

iTRAQ:

Isobaric tag for relative and absolute quantification

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Acknowledgments

This work was in part supported by grants from the National Key Technology Research and Development Program of China (2017YFD0101803; 2017YFD0101806), Jiangsu Agricultural Science and Technology Innovation Fund [JASTIF, CX(16)1012] and the Key Technology Research and Development Program of Jiangsu Province (BE2016379).

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  1. Ronghua Wang

    Present address: Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, **an, 250100, People’s Republic of China

Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nan**g Agricultural University, Nan**g, 210095, People’s Republic of China

    Ronghua Wang, Liang Xu, Yan Wang & Liwang Liu

  2. Yancheng Academy of Agricultural Sciences, Yancheng, 224002, Jiangsu, People’s Republic of China

    Yi Mei & Jun Guo

  3. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    **anwen Zhu

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Wang, R., Mei, Y., Xu, L. et al. Differential proteomic analysis reveals sequential heat stress-responsive regulatory network in radish (Raphanus sativus L.) taproot. Planta 247, 1109–1122 (2018). https://doi.org/10.1007/s00425-018-2846-5

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