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Different Morphologies and Functional Nitrogen Accumulation Results in the Different Nitrogen Use Efficiency of Tobacco Plants

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Abstract

Improving the nitrogen use efficiency in crops can significantly contribute toward reducing nutrient loss in soil and alleviating environmental pressure. It is well known that different morphologies and functional nitrogen play essential roles in the photosynthesis and respiration of crops. However, it is still unknown whether the differences in the accumulation and distribution of different morphological and functional nitrogen affect the nitrogen use efficiency in plants or not. In this study, we explored the impact of different application rates of pure nitrogen (0, 4, and 8 g/plant) on the accumulation of biomass, accumulation of nitrogen, and accumulation of different morphologies and functional nitrogen in  different organs and nitrogen use efficiency of two flue-cured tobacco cultivars (Hongda and K326). Results showed that under CK (0 g/plant) and medium (4 g/plant) application rates of nitrogen fertilizer, the biomass and nitrogen accumulation in different organs and nitrogen use efficiency of Hongda was significantly higher than K326. Although under a high application rate of nitrogen (8 g/plant), the root system development of Hongda was reduced, but its leaf biomass and nitrogen accumulation were found higher than that of K326. Analysis of accumulation of different functional nitrogen in leaves showed that Hongda had a higher capacity to accumulate carboxylation system nitrogen, respiratory system nitrogen, and photosynthetic system nitrogen, which correlates with the higher photosynthetic and respiratory efficiency in Hongda. Further pairwise correlation analysis among nitrogen use efficiency, accumulation of different morphologies and functional nitrogen in different organs showed that nitrogen accumulation in the respiratory and carboxylation systems had a strong association with nitrogen use efficiency of flue-cured tobacco plants. This study revealed that Hongda could accumulate more carboxylation and respiratory system nitrogen which can promote stronger photosynthetic and respiratory abilities, resulting in higher nitrogen using efficiency of Hongda.

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This material is the authors' own original work, which has not been previously published elsewhere and have no conflict of interest.

References

  • Anas M, Liao F, Verma KK, Sarwar MA, Mahmood A, Chen ZL, Li Q, Zeng XP, Liu Y, Li YR (2020) Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency. Biol Res 53:47. https://doi.org/10.1186/s40659-020-00312-4

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bote AD, Zana Z, Ocho FL, Vos J (2018) Analysis of coffee (Coffea arabica L.) performance in relation to radiation level and rate of nitrogen supply II. Uptake and distribution of nitrogen, leaf photosynthesis and first bean yields. Eur J Agron 92:107–114

    Article  CAS  Google Scholar 

  • Cai Q, Zhou G, Ahmed W, Cao Y, Zhao M, Li Z, Zhao Z (2021) Study on the relationship between bacterial wilt and rhizospheric microbial diversity of flue-cured tobacco cultivars. Eur J Plant Pathol 160:265–276

    Article  CAS  Google Scholar 

  • Chang D, Lai Z, Li S, Li D, Zhou J (2021) Critical source areas’ identification for non-point source pollution related to nitrogen and phosphorus in an agricultural watershed based on SWAT model. Environ Sci Pollut Res 28:47162–47181

    Article  CAS  Google Scholar 

  • Crous KY, Wallin G, Atkin OK, Uddling J, Af Ekenstam A (2017) Acclimation of light and dark respiration to experimental and seasonal warming are mediated by changes in leaf nitrogen in Eucalyptus globulus. Tree Physiol 37:1069–1083. https://doi.org/10.1093/treephys/tpx052

    Article  CAS  PubMed  Google Scholar 

  • Drake M, Vann M, Fisher L (2015) Influence of nitrogen application rate on the yield, quality, and chemical components of flue-cured tobacco, part II: application method. Tobacco Sci. https://doi.org/10.3381/14-043

    Article  Google Scholar 

  • Fageria NK, Baligar V (2005) Enhancing nitrogen use efficiency in crop plants. Adv Agron 88:97–185

    Article  CAS  Google Scholar 

  • Fan TF, He MJ, Li CJ, Shi DX, Yang C, Chen YY, Ke J, Chen YX, Zhang L, Li DQ, Liu LH, Xu C (2018) Physiological dissection revealed that both uptake and assimilation are the major components regulating different growth responses of two tobacco cultivars to nitrogen nutrition. Plant Biol (stuttg) 20:39–49. https://doi.org/10.1111/plb.12642

    Article  CAS  PubMed  Google Scholar 

  • Gao L, Lu Z, Ding L, **e K, Wang M, Ling N, Guo S (2020) Anatomically induced changes in rice leaf mesophyll conductance explain the variation in photosynthetic nitrogen use efficiency under contrasting nitrogen supply. BMC Plant Biol 20:527. https://doi.org/10.1186/s12870-020-02731-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guo R, Sun S, Liu B (2016) Difference in leaf water use efficiency/photosynthetic nitrogen use efficiency of Bt-cotton and its conventional peer. Sci Rep 6:33539. https://doi.org/10.1038/srep33539

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hanbin Z, Wanjun R, Wenyu Y, **aoyan W, Zhu W, Jizhi Y (2010) Effects of different nitrogen levels on morphological and physiological characteristics of relay-planting soybean root.

  • Hirel B, Le Gouis J, Ney B, Gallais A (2007) The challenge of improving nitrogen use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches. J Exp Bot 58:2369–2387

    Article  CAS  PubMed  Google Scholar 

  • Hou M, ** Q, Wu X, Yao Y, Zhang Z (2017) Impact of top-pruning time on the fertilizer N use efficiency of flue-cured tobacco as assessed by 15N tracing technique. Arch Agron Soil Sci 63:1324–1335

    Article  CAS  Google Scholar 

  • Hu W, Wei J, Di Q, Tao T, Zhang J, Liu J, Shi X (2021) Flue-cured tobacco (Nicotiana tabacum L.) leaf quality can be improved by grafting with potassium-efficient rootstock. Field Crops Res 274:108305

    Article  Google Scholar 

  • Imai K, Suzuki Y, Makino A, Mae T (2005) Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose 1· 5-bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence. Plant Cell Environ 28:1589–1600

    Article  CAS  Google Scholar 

  • Johnson IR, Thornley JH, Frantz JM, Bugbee B (2010) A model of canopy photosynthesis incorporating protein distribution through the canopy and its acclimation to light, temperature and CO2. Ann Bot 106:735–749

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lehmeier CA, Wild M, Schnyder H (2013) Nitrogen stress affects the turnover and size of nitrogen pools supplying leaf growth in a grass. Plant Physiol 162:2095–2105

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li Y, Yan W, Wang F, Lv S, Li Q, Yu Q (2019) Nitrogen pollution and sources in an aquatic system at an agricultural coastal area of Eastern China based on a dual-isotope approach. Environ Sci Pollut Res 26:23807–23823

    Article  CAS  Google Scholar 

  • Li Q, Ding G, Yang N, White PJ, Ye X, Cai H, Lu J, Shi L, Xu F (2020) Comparative genome and transcriptome analysis unravels key factors of nitrogen use efficiency in Brassica napus L. Plant Cell Environ 43:712–731. https://doi.org/10.1111/pce.13689

    Article  CAS  PubMed  Google Scholar 

  • Li C, Ahmed W, Li D, Yu L, Xu L, Xu T, Zhao Z (2022) Biochar suppresses bacterial wilt disease of flue-cured tobacco by improving soil health and functional diversity of rhizosphere microorganisms. Appl Soil Ecol 171:104314

    Article  Google Scholar 

  • Liang T, Wang J, Zhang Y, ** J, Zhou H, Wang B, Yin Q (2013) Spectral characteristics of tobacco cultivars with different nitrogen efficiency and its relationship with nitrogen use. International Conference on Computer and Computing Technologies in Agriculture. Springer

  • Liu T, Ren T, White PJ, Cong R, Lu J (2018) Storage nitrogen co-ordinates leaf expansion and photosynthetic capacity in winter oilseed rape. J Exp Bot 69:2995–3007

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Long SP, Bernacchi C (2003) Gas exchange measurements, what can they tell us about the underlying limitations to photosynthesis? Procedures and sources of error. J Exp Bot 54:2393–2401

    Article  CAS  PubMed  Google Scholar 

  • Lugon-Moulin N, Martin F, Krauss MR, Ramey PB, Rossi L (2006) Cadmium concentration in tobacco (Nicotiana tabacum L.) from different countries and its relationship with other elements. Chemosphere 63:1074–1086

    Article  CAS  PubMed  Google Scholar 

  • Makino A, Mae T, Ohira K (1984) Relation between nitrogen and ribulose-1, 5-bisphosphate carboxylase in rice leaves from emergence through senescence. Plant Cell Physiol 25:429–437

    CAS  Google Scholar 

  • Marchetti R, Castelli F, Contillo R (2006) Nitrogen requirements for flue-cured tobacco. Agron J 98:666–674

    Article  CAS  Google Scholar 

  • Pao YC, Chen TW, Moualeu-Ngangue DP, Stützel H (2019) Environmental triggers for photosynthetic protein turnover determine the optimal nitrogen distribution and partitioning in the canopy. J Exp Bot 70:2419–2434. https://doi.org/10.1093/jxb/ery308

    Article  CAS  PubMed  Google Scholar 

  • Parker RG (2008) Evaluation of nitrogen sources and rates on yield and quality of modern flue-cured tobacco cultivars. North Carolina State University.

  • Pask A, Sylvester-Bradley R, Jamieson P, Foulkes M (2012) Quantifying how winter wheat crops accumulate and use nitrogen reserves during growth. Field Crop Res 126:104–118

    Article  Google Scholar 

  • Perchlik M, Tegeder M (2018) Leaf amino acid supply affects photosynthetic and plant nitrogen use efficiency under nitrogen stress. Plant Physiol 178:174–188. https://doi.org/10.1104/pp.18.00597

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Porra R, Thompson WA, Kriedemann P (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim. Biophys. Acta - Bioenerg. 975:384–394

    Article  CAS  Google Scholar 

  • Richards R (2000) Selectable traits to increase crop photosynthesis and yield of grain crops. J Exp Bot 51:447–458

    Article  CAS  PubMed  Google Scholar 

  • Shi D-y LI, Y-h ZHANG J-w, Peng L, Bin Z, S-t DONG (2016) Increased plant density and reduced N rate lead to more grain yield and higher resource utilization in summer maize. J Integr Agric 15:2515–2528

    Article  Google Scholar 

  • Sirong Z, Siyu G, Shichuan Z, Renxiao C, Qiyuan L, **aoqing Z (2018) Research progress on low nitrogen tolerance and nitrogen efficiency in crop plants. J Nuclear Agric Sci 32:1656

    Google Scholar 

  • Song R, Ahmed W, Tan Y, Zhao Z (2022) Different levels of nitrogen fertilizer in nursery stage positively affect the activity of defense-related enzymes and resistance of tobacco plant to Phytophthora nicotianae. Chiang Mai J Sci 49(3):551–554

    Article  CAS  Google Scholar 

  • Su J, Chen Y, Zhu Y, **ang J, Chen Y, Hu B, Zhu M, Xu A, Li F, Ren K (2021) The response of Hongda, a flue-cured tobacco cultivar, to nitrogen fertilizer rate. Arch Agron Soil Sci 67:536–550

    Article  CAS  Google Scholar 

  • Sun Y, Tong C, He S, Wang K, Chen L (2018) Identification of nitrogen, phosphorus, and potassium deficiencies based on temporal dynamics of leaf morphology and color. Sustainability 10:762

    Article  Google Scholar 

  • Takashima T, Hikosaka K, Hirose T (2004) Photosynthesis or persistence: nitrogen allocation in leaves of evergreen and deciduous Quercus species. Plant, Cell Environ 27:1047–1054

    Article  CAS  Google Scholar 

  • Tang Z, Chen L, Chen Z, Fu Y, Sun X, Wang B, **a T (2020) Climatic factors determine the yield and quality of Honghe flue-cured tobacco. Sci Rep 10:1–12

    Article  Google Scholar 

  • Thomas R, Sheard R, Moyer J (1967) Comparison of conventional and automated procedures for nitrogen, phosphorus, and potassium analysis of plant material using a single digestion 1. Agron J 59:240–243

    Article  CAS  Google Scholar 

  • Urban A, Rogowski P, Wasilewska-Dębowska W, Romanowska E (2021) Understanding maize response to nitrogen limitation in different light conditions for the improvement of photosynthesis. Plants 10:1932

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Walter J, Kreyling J, Singh BK, Jentsch A (2016) Effects of extreme weather events and legume presence on mycorrhization of Plantago lanceolata and Holcus lanatus in the field. Plant Biol 18:262–270

    Article  CAS  PubMed  Google Scholar 

  • Wang YY, Cheng YH, Chen KE, Tsay YF (2018) Nitrate transport, signaling, and use efficiency. Annu Rev Plant Biol 69:85–122. https://doi.org/10.1146/annurev-arplant-042817-040056

    Article  CAS  PubMed  Google Scholar 

  • Wang F, Ge S, Xu X, **ng Y, Du X, Zhang X, Lv M, Liu J, Zhu Z, Jiang Y (2021) Multiomics analysis reveals new insights into the apple fruit quality decline under high nitrogen conditions. J Agric Food Chem 69:5559–5572

    Article  CAS  PubMed  Google Scholar 

  • Xu G, Fan X, Miller AJ (2012) Plant nitrogen assimilation and use efficiency. Annu Rev Plant Biol 63:153–182

    Article  CAS  PubMed  Google Scholar 

  • Yang L, Wei Z, Valls M, Ding W (2021) Metabolic profiling of resistant and susceptible tobaccos response incited by ralstonia pseudosolanacearum causing bacterial Wilt. Front Plant Sci 12:780429

    Article  PubMed  Google Scholar 

  • Yang R, Yang J, Yu J, Wang S, Yang C, Xu F (2022) Effects of different nitrogen application rates on the quality and metabolomics of cigar tobacco. Agron J 114:1155–1167

    Article  CAS  Google Scholar 

  • Zhang H-Y, Liu X-Z, Li T-S, Yang Y-M (2006) Genetic diversity among flue-cured tobacco (Nicotiana tabacum L.) revealed by amplified fragment length polymorphism. Bot Stud 47:223–229

    CAS  Google Scholar 

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Acknowledgements

This study was financially supported by the Yunnan Agricultural University Scientific Research Foundation (KX900187) and the Science & Technology Platform Plan of Yunnan Province (2019IC005).

Funding

Yunnan Agricultural University Scientific Research Foundation (KX900187) and Science & Technology Platform Plan of Yunnan Province (2019IC005).

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Authors and Affiliations

  1. College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Shichen Li

  2. Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Waqar Ahmed, Tao Zhang, Tao Jiang, Fupeng Mei, Qu Shan, Linyuan Yang, Cuilian Guo & Zhengxiong Zhao

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  1. Shichen Li
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  9. Zhengxiong Zhao
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Contributions

ZZ conceived and designed the experiments. SL, TZ, TJ, and FM performed the experiments. SL, FM, QS, LY, and CG collected and analyzed the data. SL and WA wrote the manuscript. WA and ZZ revised the manuscript. All authors contributed to the final draft of the manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Zhengxiong Zhao.

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Li, S., Ahmed, W., Zhang, T. et al. Different Morphologies and Functional Nitrogen Accumulation Results in the Different Nitrogen Use Efficiency of Tobacco Plants. J Plant Growth Regul 42, 5895–5908 (2023). https://doi.org/10.1007/s00344-023-10975-4

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