Abstract
The triterpenes have low solubility, as a strategy to facility its transport into the gastro-intestinal tract, the techno-functional properties of the mixture of high degree of polymerization Agave fructans (HDPAF) and whey protein (WP) as encapsulating material for stabilized Coccoloba uvifera L. extract fraction were evaluated. The purification of the extract was carried out by column chromatography, obtaining the fraction with the highest bioactivity. The microstructure evaluated by SEM showed capsules with spherical shapes using electrospraying. HPLC–MS analysis indicated the presence of β-sitosterol and lupeol in the encapsulates. The main interactions of biopolymers and compounds were by FTIR. The Tg of the capsules (> 149 °C) and the thermal stability (> 202 °C) of the compounds were by thermal analysis (DSC and TGA, respectively). The loading and encapsulation efficiency were 100 and 95%, respectively. Finally, the encapsulates presented antioxidant capacities for ABTS, DPPH, and FIC (between 90 and 71%). The HDPAF-WP mixture has techno-functional properties that allow the stabilization of HBVC by electrospraying.
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Acknowledgements
The authors thank Tecnológico Nacional de México for their support in conducting the work throughout the project “Fracciones de extracto de hoja de uva de mar (Coccoloba uvifera L.): Evaluación de la actividad biológica y encapsulación por electroesprayado” (code 11176.21-P), Consejo Nacional de Ciencia y Tecnología (CONACyT, Mexico) for the scholarship granted (number 702624) to Jorge Alberto Ramos-Hernández; CYTED thematic network code 319RT0576 and Spanish Ministry of Science, Innovation and Universities (project code RTI2018-097249-B-C21). The author's thanks Claudia Hernández Ambrosio for technical assistance.
Funding
Tecnológico Nacional de México for their support in conducting the work throughout this project: Fracciones de extracto de hoja de uva de mar (Coccoloba uvifera L.): Evaluación de la actividad biológica y encapsulación por electroesprayado, CONACYT (Mexico) for the scholarship granted (number 467459).
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Jorge A. Ramos-Hernández: Conceptualization, Methodology, Formal analysis, Investigation, Data curation. Montserrat Calderón-Santoyo: Conceptualization, Methodology, Writing—review & editing. Cristina Prieto: Methodology, Data curation, Review & editing. José M. Lagarón: Methodology, Review & editing. Arturo Nvarro-Ocaña: Methodology, Review & editing. Juan Arturo Ragazzo-Sánchez: Conceptualization, Methodology, Project administration, Supervision, Writing—review & editing.
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Ramos-Hernández, J.A., Calderón-Santoyo, M., Prieto, C. et al. Encapsulation with HDPAF-WP of the hexane fraction of sea grape (Coccoloba uvifera L.) leaf extract by electrospraying. Polym. Bull. 80, 959–975 (2023). https://doi.org/10.1007/s00289-022-04088-3
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DOI: https://doi.org/10.1007/s00289-022-04088-3