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Bacillus licheniformis and Bacillus velezensis from Rhizosphere of Clerodendrum infortunatum L. Promote Plant Growth and Resistance to Sclerotium rolfsii in Vigna unguiculata (L.) Walp.

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Abstract

In the current study, thirty bacterial strains isolated from the rhizosphere of Clerodendrum infortunatum L. were evaluated for the properties related to the plant growth promotion and disease resistance. Here, all the selected strains were screened for its antagonistic effect towards the phytopathogen Sclerotium rolfsii and also for the production of bioactive compounds known to promote the plant growth. Among these isolates, CiRb1 and CiRb16 were observed to have a broad range of plant beneficial features and were identified as Bacillus licheniformis and Bacillus velezensis respectively. Both the isolates were also demonstrated to produce the volatile organic compounds (VOCs) responsible for the growth enhancement in Brassica nigra (L.) and growth inhibition of S. rolfsii. Talc based formulations made out of both B. licheniformis and B. velezensis were further demonstrated to augment the plant growth and protection against S. rolfsii in Vigna unguiculata (L.) Walp. By the GC–MS based analysis, undecane could also be detected in the methanolic extracts prepared from both B. licheniformis and B. velezensis. Here, the selected rhizobacterial isolates were found to promote the plant growth and disease resistance through both direct and VOC mediated mechanisms. The results of the study hence reveal both B. licheniformis and B. velezensis have the potential in field application to promote the growth and control of plant diseases.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors acknowledge Kerala State Council for Science, Technology and Environment (KSCSTE)-Science Research Scheme (SRS) for the financial support and JAIVAM and KSCSTE- KBC- YIPB project for the support. The authors also acknowledge the Department of Science and Technology under DST- PURSE for the GC-MS instrumentation facility.

Funding

Funding received from Kerala State Council for Science, Technology and Environment (KSCSTE)-Science Research Scheme (SRS) during the study.

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Authors and Affiliations

  1. School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686 560, India

    Jishma Panichikkal, Smruthy Manu & Radhakrishnan Edayileveetil Krishnankutty

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Contributions

Radhakrishnan Edayileveetil Krishnankutty contributed to the study conception and design. Experiments and analyses were performed by Jishma Panichikkal and Smruthy Manu. The first draft of the manuscript was written by Jishma Panichikkal and Radhakrishnan Edayileveetil Krishnankutty. The manuscript was revised and corrections were included by Radhakrishnan Edayileveetil Krishnankutty. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Radhakrishnan Edayileveetil Krishnankutty.

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Panichikkal, J., Manu, S. & Krishnankutty, R.E. Bacillus licheniformis and Bacillus velezensis from Rhizosphere of Clerodendrum infortunatum L. Promote Plant Growth and Resistance to Sclerotium rolfsii in Vigna unguiculata (L.) Walp.. Curr Microbiol 81, 238 (2024). https://doi.org/10.1007/s00284-024-03749-y

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