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Bacillus litorisediminis sp. nov., a Thermophilic Bacterium Isolated from Mangrove Sediment

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Abstract

Two aerobic, Gram-staining-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-47801T and FJAT-47835, were isolated from the sediment collected from Zhangjiang Estuary Mangrove National Nature Reserve in Fujian Province, China. Growth was observed at 25–55 °C (optimum, 50 °C) and pH 7.0–9.0 (optimum, pH 7.0), with up to 4.0% (w/v) NaCl (optimum, without NaCl). Strains FJAT-47801T and FJAT-47835 showed the highest 16S rRNA gene sequence similarity to Bacillus oleivorans (98.5%). The 16S rRNA gene sequence similarity between FJAT-47801T and FJAT-47835 was 99.9% indicating they were the same species. Phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (based on 120 conserved bacterial single-copy genes) trees showed that strains FJAT-47801T and FJAT-47835 should be affiliated to the genus Bacillus. The of menaquinone of strain FJAT-47801T was MK-7. The major fatty acids of strain FJAT-47801T were iso-C15:0, anteiso-C15:0, iso-C17:0, and C16:0. The major polar lipids strain FJAT-47801T were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), and phosphatidylglycerol (PG). The genomic DNA G+C content of strain FJAT-47801T was 39.3%. The average nucleotide identity (84.3%) and the digital DNA–DNA hybridization value (28.1%) between strain FJAT-47801T and B. oleivorans CCTCC AB 2013353T were below the cut-off level for species delineation. Based on the above results, strain FJAT-47801T represents a novel species of the genus Bacillus, for which the name Bacillus litorisediminis sp. nov., is proposed. The type strain is FJAT-47801T (=GDMCC 1.2712T = JCM 34875T).

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Data Availability

The 16S rRNA gene and genome sequences of strain FJAT-47801T have been deposited in GenBank under accession numbers OM857626 and JAKZKT000000000, respectively.

Code Availability

Not applicable.

Abbreviations

ANI:

Average Nucleotide Identity

dDDH:

Digital DNA–DNA hybridization

PE:

Phosphatidylethanolamine

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

UAPL:

Unidentified amino phospholipid

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Acknowledgements

We thank Prof. A. Oren for his suggestion on nomenclature.

Funding

This work was financially supported by the National Natural Science Foundation of China (42007221 and GJYS202203).

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Author notes

  1. Rong Tang and Shang Yang have contributed equally to this work.

Authors and Affiliations

  1. Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, People’s Republic of China

    Rong Tang, Shang Yang, Shuang Han, Cheng-Jie **e & Shun-Gui Zhou

  2. Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350003, People’s Republic of China

    Guo-Hong Liu

  3. Administrative Bureau of Zhangjiang Estuary Mangrove, National Nature Reserve Yunxiao Town, Yunxiao, Fujian, 363300, People’s Republic of China

    Guan-Min Huang

  4. Programa de Doctorado en Ciencias Aplicadas, Universidad Autónoma de Chile, Talca, 3460000, Chile

    Manik Prabhu Narsing Rao

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Contributions

RT and SY carried out the experiments and wrote the manuscript. SH and C-J X performed genome analysis. G-M H provided samples. MPNR performed genome analysis and revised the manuscript. G-H L and S-G Z supervised the study.

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Correspondence to Guo-Hong Liu or Shun-Gui Zhou.

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Tang, R., Yang, S., Han, S. et al. Bacillus litorisediminis sp. nov., a Thermophilic Bacterium Isolated from Mangrove Sediment. Curr Microbiol 80, 79 (2023). https://doi.org/10.1007/s00284-023-03180-9

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