Abstract
White-rot fungi are the main decomposers of wood cell-wall polymer in forest ecosystems. Little is known, however, about the interactions between white-rot fungi and other coexisting microorganisms in decayed wood. A white-rot fungus, Trametes versicolor strain TN6F, was isolated from a fruit body, and 44 strains of coexisting cultivable bacteria were isolated from its substrate, natural white rot-decayed wood. The effects of these bacteria on fungal growth were examined by an in vitro confrontation growth assay. Among the isolates, nine bacterial strains inhibited the growth of strain TN6F, while 35 strains did not affect the growth of TN6F. However, when co-cultured with strain TN6F on wood powder, many bacterial strains promoted the weight loss of the substrate. A subsequent chemical composition analysis showed that co-culturing accelerated delignification. Higher laccase activity was detected when strain TN6F was co-cultured on wood powder medium with bacterial strains TN6W-26 or TN6W-27. These results indicate that some bacterial strains might promote wood degradation.
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Acknowledgements
The author would like to thank Mai Komatsuo, Ryuji Hori, and Kiyomatsu Ryohei for technical assistance with the experiments. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant Nos. 23688041 and 15K14905).
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Kamei, I. Co-culturing Effects of Coexisting Bacteria on Wood Degradation by Trametes versicolor . Curr Microbiol 74, 125–131 (2017). https://doi.org/10.1007/s00284-016-1162-1
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DOI: https://doi.org/10.1007/s00284-016-1162-1