Abstract
Conversion of crude glycerol derived from biodiesel processes to value-added chemicals has attracted much attention. Herein, Raoultella ornithinolytica B6 was investigated for the high production of 2,3-butanediol (2,3-BD) from glycerol without 1,3-propanediol (1,3-PD) formation, a by-product hindering 2,3-BD purification. By evaluating the effects of temperature, agitation speed, and pH control strategy, the fermentation conditions favoring 2,3-BD production were found to be 25 °C, 400 rpm, and pH control with a lower limit of 5.5, respectively. Notably, significant pH fluctuations which positively affect 2,3-BD production were generated by simply controlling the lower pH limit at 5.5. In fed-batch fermentation under those conditions, R. ornithinolytica B6 produced 2,3-BD up to 79.25 g/L, and further enhancement of 2,3-BD production (89.45 g/L) was achieved by overexpressing homologous 2,3-BD synthesis genes (the budABC). When pretreated crude glycerol was used as a sole carbon source, R. ornithinolytica B6 overexpressing budABC produced 78.10 g/L of 2,3-BD with the yield of 0.42 g/g and the productivity of 0.62 g/L/h. The 2,3-BD titer, yield, and productivity values obtained in this study are the highest 2,3-BD production from glycerol among 1,3-PD synthesis-deficient 2,3-BD producers, demonstrating R. ornithinolytica B6 as a promising 2,3-BD producer from glycerol.
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Acknowledgements
This work was supported by a National Research Council of Science and Technology (NST) grant by the Korea government (MSIP) (No. CAP-11-04-KIST). The authors also appreciate the support from the Korea Institute of Energy Technology Evaluation and Planning (Project No. 20143010091880) and C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (2016M3D3A1A01913249).
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Kim, T., Cho, S., Woo, H.M. et al. High production of 2,3-butanediol from glycerol without 1,3-propanediol formation by Raoultella ornithinolytica B6. Appl Microbiol Biotechnol 101, 2821–2830 (2017). https://doi.org/10.1007/s00253-017-8094-y
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DOI: https://doi.org/10.1007/s00253-017-8094-y